结合表型生长及分子指纹图谱构建亚美马褂木无性系档案-森林培育专业论文.docxVIP

106个等位基因，无性系群体平均观察杂合度Obs．Het为0．5628，范围为0-0．9545；平均期望杂合度Exp 106个等位基因，无性系群体平均观察杂合度Obs．Het为0．5628，范围为 0-0．9545；平均期望杂合度Exp Het为0．6260，范围为0．41 94—0．7410；多样 性指数Nei平均值为0．6223，范围为0．4169—0．7368。 (4)利用27对引物构建亚美马褂木指纹条码，再从27对引物中选出 多态性高、有特异标记的002、015、056、113、173和LT21共6对引物作 为核心引物区分鉴别20个无性系，并构建指纹图谱。编号为1，3，7，8， 9，10，11，12，14，1 5，l 8，19，20共12个无性系有自己特异的等位基 因。 (5)结合20个亚美马褂木无性系的表型生长及分子指纹图谱分析发 现，20个无性系可分为两大类，2，11，10，20，9，12，3，5，17，19共11个无性系组 合为一类，表现出优良的用材树种特性；14，15，16，18，1，6，17，4，8共9个无性 系组合为另一类，表现出相对较差的用材树种特性。 关键词：亚美马褂木表型性状SSR分子标记指纹图谱 万方数据 COMBINED COMBINED WITH PHENOTYPIC GROWTH AND MOLECULAR FINGERPRINT CONSTRUCTION OF LIRIODENDRON SINO．AMERANUM CLONES ARCHIVES ABSTRAC T Liriodendron sino—americanum is an artificial hybrid species，which was crossed from Liriodendron chinense Sarg and Liriodendron tu@ifera．L．It has been proved to possess an obvious heterosis and was an excellent species for timber harvest and garden planting．In this study,the growth performance of twenty L．sino．americanum clones，which were planted in Nanning，Guangxi province 5 years before，were evaluated．The genetic diversity detection and clonal fingerprints were completed by S SR molecular markers．Finally,the comprehensive clonal archives were set up through combining phenotypic traits with clonal fingerprints．It will be an important reference and guidance for subsequent application of all tested clones．The main results were as follows： (1)The ANOVA results showed that 1 3 of 1 5 traits were extremely significant difference(PO．0 1)among clones．The ranges of various characters， genetic variation coefficient and repeatability were 10．6％～1 09．59％，2．55％ 24．1 0％and O．1 373～O．1 373，respectively．That showed the genetic variation between clones was relatively abundant，and the traits tested was strongly under thegenetic contr01． (2)With the aim for timber cultivation，principal component analysis and IlI 万方数据 comprehensive comprehensive index evaluation were carried out for clone selection．The preferred clones were No