金属铜纳米颗粒的吸入毒性和脑损伤研究-生物化学与分子生物学专业论文.docxVIP

PAGE PAGE III Inhaled Toxicity and Brain Damage of Copper Nanoparticles Major: Biochemistry and Molecular Biology Candidate for M.A: Liu Yang Directed by: Prof. WU Gang Prof. CHEN Chunying ABSTRACT OBJECTIVE：We will different doses and sized copper particles exposed to mice via nasal instillation, nano-sized copper particless overall toxicity on mice as well as the retention and distribution of copper particles, along with copper particles arosed brain damage of mice were researched. Our experiment primary discussed biological effect and damage mechanism after inhaled copper particles. METHODS：To assess nano-sized copper particless overall toxicity on mice as well as the retention and distribution of copper particles, the high sensitive ICP-MS was employed to detect the contents of copper in tissues, use histopathological method to examination morphological changes of main tissues and visceras, utilize blood biomarker assay results to discusse damage mechanism. The SRXRF will analysis content copper in olfactory bulb and brain tissues. Detect oxidative stress correlative index to speculate possible mechanism. Special protein expression of immunohisto -chemistry to detect damage degree of brain tissue. Use TEM to observe ultrastructure of nerve cell in olfactory bulb and brain tissues. RESULTS：The ICP-MS results indicate there were significant increase of copper content in the liver, kidneys and olfactory bulb. The histopathological photomicrographs indicate the serious swelling in the renal glomerulus was observed . In the liver tissue of the experimental mice, the hydropic degeneration around the central vein was prominent. The olfactory cells had severe lesion and quantity of olfactory cells decreased obviously, the samdwich of olfactory was dilapidated. The SRXRF detect found contents of copper in olfactory bulb and brain tissues were significant increases, morover, incresesed area of copper content were extensive. There were not significant increases in th