双光子显微成像技术的新进展-中国药物警戒.PDF

Chinese Journal of Medical Instrumentation 2011 年35 卷第3 期 综 合 评 述 文章编号 1671-7104(2011)03-0204-05 ： 双光子显微成像技术的新进展 夏伟强1 2* 2 【作 者】 ，周源 ，石明 1 北京航空航天大学仪器科学与光电工程学院，北京，100191 2 河北承德实验中学，河北，承德，167101 【摘 要】双光子显微成像技术是近年发展起来的一种新型非线性光学成像方法,由于其优异的特性已广泛用于细胞生物活细胞、组织 的长时间动态三维成像。文中首先介绍了双光子成像的原理和特点；在此基础上总结了双光子显微成像技术的研究热点； 其次并分别就如何获得更低的细胞损害、更快的图像获取速度和更高的成像灵敏度三个方面作了论述；最后展望了双光子 显微成像技术的发展前景。 【关 键 词】双光子；全内反射；活细胞成像；三维成像；光致漂白 【中图分类号】Q693 【文献标志码】A doi:10.3969/j .issn.1671-7104.2011.03.011 Advances in Two-photon Imaging Technology 【Writers 】 1 2 2 Xia Weiqiang , ZhouYuan , Shi1 Ming 1 School of Instrumentation Science and Optoelectronics Engineering, Beij ing University of Aeronautics and Astronautics, Beij ing, 100191, China 2 Hebei Chengde Experiment Middle School, Chengde, Hebei, 167101, China 【Abstract 】As a new kind of advanced nonlinear imaging approach, two-photon fluorescence microscopy technology is wildly used in the field of live cell and tissue imaging ，especially focusing on long-term dynamic three-dimensional cell imaging. This paper firstly presents the principle and characteristic of two-photon fluorescence microscopy. Then, the paper focuses on the three key aspects of the viability of the specimen, sensitivity of detection, as well as the speed of acquisition. In the end, the future prospect of development and application of two-phonon imaging technology are predicted 【 】 Key words two-photon imaging, total Internal reflection imaging, live cell imaging, 3-D cell imaging, photo bleaching 表达的原因 从而帮助生物学家及生理学家总结出 0 引言 ，