薏苡和夏枯草种质资源生物学特性和遗传多样性的研究与评价.pdfVIP

3．2．3．2琼脂皿法………………………………………………………………………16 3．2．4含水量测定……………………………………………………………………．．16 3．2．5生活力测定……………………………………………………………………．16 3．2．6发芽率测定……………………………………………………………………．16 3．2．7生物学特性及农艺性状观测…………………………………………………．17 3．2．8提取基因组DNA………………………………………………………………17 3．2．9 AFLP分析…………………………………………………………………………………………20 3．2．9．1酶切…………………………………………………………………………………………………．．20 3．2．9．2连接…………………………………………………………………………………………………．．21 3．2．9．3预扩增………………………………………………………………………．．21 3．2．9．4选择性扩增…………………………………………………………………．．21 3．2．9．5变性聚丙烯酰胺凝胶电泳…………………………………………………．．2l 4结果与分析………………………………………………………………………。24 4．1薏苡种子特性分析………………………………………………………………24 4．1．1薏苡种子净度……………………………………………………………………24 4．1．2薏苡种子千粒重………………………………………………………………26 4．1．5薏苡种子生活力………………………………………………………………27 4．1．6薏苡种子发芽率………………………………………………………………28 4．2薏苡生物学特性分析……………………………………………………………29 4．3薏苡遗传多样性分析……………………………………………………………3l 4．3．1薏苡DNA的提取和质量分析………………………………………………．31 4．3．2薏苡AFLP指纹图谱的建立和鉴别效率……………………………………31 4．3．312个薏苡种质的AFLP指纹图谱……………………………………………34 4．3．4基于AFLP分子标记的薏苡种质分类………………………………………．34 4．4夏枯草种子特性分析……………………………………………………………35 4．5夏枯草生物学特性分析…………………………………………………………36 4．6夏枯草遗传多样性分析…………………………………………………………37 4．6．1夏枯草DNA的提取和质量分析……………………………………………一37 4．6．2夏枯草AFLP指纹图谱的建立和鉴别效率…………………………………38 ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ■ ● ● 40 ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● 4O ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● 41 参考文献………………………………………………………………………………43 作者简介………………………………………………………………………………47