基于ISSRPCR对山东玉米多堆柄锈菌遗传多样性的研究及其初侵染菌源的推测.docx

基于ISSR-PCR对山东玉米多堆柄锈菌 遗传多样性的研究及其初侵染菌源的推 鄢洪海 王琰 张茹琴 夏淑春 王志奎 宋希云 青岛农业大学植物医学学院山东省应用真菌重点实 验室 山东省乳山市农业局 即墨市农业局 摘要： 利用11对多态性ISSR引物对2014-2016年间采自山东、江苏、浙江、河南及 海南等5省的60个玉米多堆柄锈菌菌株进行了种群遗传多样性分析。结果表明, 分离自山东的玉米南方锈菌菌株的遗传多样性丰富，多态性百分率(P)达 96.34%,与江苏、浙江、河南锈菌种群具有较高的相似性，遗传距离相对较近， 其中与江苏的群体遗传相似系数最高(GS二0.7829),遗传距离最近 (GD=0. 2447),而与分离自海南的锈菌种群相似性最低(GS=0.0148),遗传距 离最远(GD=4. 2999)。在相似性系数0. 74水平上，供试60个菌株被划为3个 群和多个亚群组，齐群和亚群间表现出较明显的年度与地理分布上的差异。2015 年采集的菌株之间Gst值为0. 8694, 2015与2016年采集的菌株群体间的Gst 值为0. 4562,说明病菌的遗传变异不仅表现在地域上，也体现在年度间。基于 TSSR标记分析，作者认为发生在山东省的玉米南方锈病其初侵染菌源不是来自 我国周年发生玉米南方锈病的海南省，而最有可能來自同样能越冬的菲律宾或 我国台湾地区。 关键词： 玉米多堆柄锈菌;山东群体;遗传多样性;ISSR; 作者简介：SONG Xi-Yun, E-mail: songxy@qau. edu. on 收稿日期：2017-05-10 基金：山东省自然科学基金(ZR2011CL005) Genetic diversity and deduction of primary infection source of Puccinia polysora in Shandong Province based on ISSR-PCR YAN Hong-Hai WANG Yan ZHANG Ru-Qin XIA Shu-Chun WANG Zhi-Kui SONG Xi-Yun College of Plant Hea1th and Medicine, Qingdao Agricultural University, Sandong Province Key Laborstory of Applied Mycology; Shandong Rushan City Agriculture Bureau; Jimo Agriculture Bureau; Abstract： The genetic diversity of 60 strains of Puccinia polysora collccted from Shandong, Jiangsu, Zhejiang, Henan and Hainan provinces from 2014 to 2016 was analyzed by using 11 pair of polymorphic 1SSR primers. The results indicated that, the genetic diversity of the strains collected from Shandong Province was abundant, and its polym orphic percentage (P) was 96.34%. The genetic diversity of the strains was highly similar to that of the st rains collected from Jiangsu, Zhejiang and Henan provinces, and the genetic distance was relatively high. The strains collected from Shandong Province showed the highest gene tic similarity coefficient (GS 二 0. 7829) and the closest genetic distanee (GD= 0. 2447) as compared to those collected from Jiangsu Province; however, the strains from Shandong showed the lowcst genetic similarity coefficient (GS= 0. 0148) and the farthest genetic distance (GD= 4. 2999