shRNA沉默c-Myc基因对胶质瘤细胞增殖及VEGF表达的影响研究.pdf

英文摘要 the andthe of Effectson expression proliferation in cellsafter the VEGF glioblastomasilencing shRNA oncogenec-Mycthrough ABSTRACT Objective： This wasto the study constructshRNA plasmidtargeting the andsliencethe of oncogenec-Myc expressionc-Mycgene inhuman celllinesIN500Acells RNA glioblastoma using interference wasalsotO technique．Then，thisstudy approach the on effectsof c-Myconcogeneglioma and bothin andin vivo vitro．Meanwhile，this apoptosis study detectedtheinfluenceonthe ofVEGFinIN500A expression cellsafter the anddiscussedthe silencingc-Myc oncogene of in anditsroleonthe c—Myc position geneglioma regulation of datawas angiogenesis．Finally,significantexperimental the for and to providedgene therapy therapyanti—angiogenesis gliomas． ． Methods： 1 ConstructionofshRNA andcelltransfection：