IGF-1对大鼠局灶性脑缺血再灌注后VEGF、bFGF表达的影响及意义.pdf

英文摘要 Cerebral was with0．01M ischemia／reperfusiongroup treated 0uLinthe same phosphate—bufferedsaline(PBS)1 way．Tim sham was treatedwith operatedgrouponly neckskinincision andvascular andwasn’testablished into separation MCAO，but itwasectedPBSinthesamevolume． inj 3．Withinthetime ratsineach were prescribed，the group sacrificed andtheirbrainswere quickly bydecapitation four removed．Thebraintissuewascutinto coronalslicesfrom antinionto were and A、B、C Dbrainslices． lobe，which occipital Abrainslicewasabandonded．Withinthetime cerebralcoaextissuearoundtheinfarctionwas regionstripped fromBandCbrainslicesandthe tissuewasabout100 stripped it inl％DEPCwaterand itintothe mg．Rinsed briefly put frozen tube and in the quicklypreserveliquidnitrogen．Detect contentof mRNAof VEGF-mRNAandbFGF Cbrainslice by RT-PCRmethod．Bbrainsliceswere for futureuse．B preserved brainslicewasusedtodetectbrain—VEGFmRNA