PMMA局部注射对兔股骨头正常组织影响的实验研究.pdf

英文摘要 Conventional ectionofanesthesia． intraperitonealinj disinfection．Medianabdominal thesmall incision，draw theabdominalaortaandinferiorvena intestine，revealed cava， insert saline， polyethylenepipecontainingheparin．Lavageby red theinferiorvenacavaintubationfirstoutflowinthe blood， and Canbe whenthecolorbe that Mopped pink．Perfusion withinkdon’t untilthecolorof stop limbsandtailbeblack．Animalsare whose died，and femoralheadcutfor bilateral be specimens．4．HEdyeing：Cut the neckatthebasementwhichbe in10％ femoral preserved for themin8％formicacidsolution formalin，marked，and put two threetimesto need demineralized，itweeks．Dewatering． the 51ma． wax．．Repairspecimen．slice Transparent．Steeping Waterbath．Stretched slice．H．E preparation．Bakedyeing： Fuse with withUrolen wax．Hydrationalcoh01．Dyeing witll Blue．Acidation．Return eosin． orchid．Dyeing withalcohol with Hydration Obse