凹叶厚朴快繁技术体系的建立.PDF

中草药 Chinese Traditional and Herbal Drugs 第 45 卷 第 12 期 2014 年 6 月 ·1769 · 凹叶厚朴快繁技术体系的建立 1 1 1 1 2 1 马英姿 ，许 欢 ，王志毅 ，刘江海 ，王晓明 ，蒋丽娟 1. 中南林业科技大学生命科学与技术学院，湖南 长沙 410004 2. 中国林科院湖南省分院无性系重点实验室，湖南 长沙 410004 摘 要：目的 建立凹叶厚朴 Magnolia officinalis subsp. biloba 快速繁殖体系。方法 以选育的优质凹叶厚朴种胚为初始外 植体，采用单因素及正交试验筛选不同基本培养基，不同植物调节剂（6-BA、NAA 、IBA、IAA ），获取最适种胚启动培养基， 丛芽增殖培养基和生根培养基。结果 适宜种胚萌发的启动培养基为 B5 ＋6-BA 0.5 mg/L＋NAA 0.1 mg/L ，出芽率达 87.0%； 适合丛芽增殖的培养基为 MS ＋6-BA 2.5 mg/L ＋NAA 0.5 mg/L ，增殖系数可达 6.2；适合生根的培养基为 1/2 MS＋NAA 0.5 mg/L ，30 d 生根率可达 84%。结论 建立了凹叶厚朴快速繁殖体系，为凹叶厚朴优质种苗的工厂化育苗奠定了基础。 关键词：凹叶厚朴；组织培养；快速繁殖；培养基；正交试验 中图分类号：R282.21 文献标志码：A 文章编号：0253 - 2670(2014)12 - 1769 - 06 DOI: 10.7501/j.issn.0253-2670.2014.12.023 Establishment of rapid propagation system of Magnolia officinalis subsp. biloba 1 1 1 1 2 1 MA Ying-zi , XU Huan , WANG Zhi-yi , LIU Jiang-hai , WANG Xiao-ming , JIANG LI-juan 1. College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China 2. Key Laboratory of Clones, Hunan Academy of Forestry, Changsha 410004, China Abstract: Objective To establish a rapid propagation system of Magnolia officinalis subsp. biloba. Methods Using peeling seeds of M . officinalis subsp. biloba as the initial explants, different media and various combinations of plant growth regulators (6-BA, NAA, IBA, and IAA) on the seeds germination, the seedlings subculture, and rooting culture were studied by single factor test and orthogonal test. Results The best culture medium of seeds germination was B5 + 6-BA 0.5 mg/L + NAA 0.1 mg/L, and the budding percentage was 87.0%; The effective medium for cluster buds-inducing and subculture was MS + 6-BA 2.5 mg/L + NAA 0.5 mg/L, and the propagation coefficient was 6.2; The