2型糖尿病大鼠心肌纤维化和心肌AGEs-中国试验动物学报.DOC

糖尿病大鼠心肌纤维化与心肌AGEs/RAGE水平的变化研究 侯改霞1，肖国强2，习雪峰1，刘倩倩1 （1. 河南大学体育学院，河南 开封475001；2. 华南师范大学体育科学学院，广东 广州510006） 【摘要】目的 通过构建Ⅱ型糖尿病大鼠模型，研究该模型心肌纤维化和心肌AGEs/RAGE水平的变化情况。方法 雄性SD大鼠高脂饲料饲喂6周后，过夜空腹12h，腹腔注射小剂量链脲佐菌素（STZ，30mg/kg）。注射3天和7天后检测大鼠随机血糖浓度，两次随机血糖≥16.7mmol/L为Ⅱ型糖尿病大鼠建模成功。在建模成功后第4、8和12周，检测大鼠血FBG、Insulin和左心室AGEs、Hyp含量及Masson染色、RAGE表达的变化。结果 造模成功后第4、8和12周，糖尿病对照组（4D，8D，12D）血FBG水平均较同周次正常对照组（4C，8C，12C）显著升高，体重显著下降；造模成功后第12周时，糖尿病对照组（12D）大鼠Insulin显著高于同周次正常对照组（12C）和4周时糖尿病对照组（4D）；从造模成功后第8周开始，糖尿病对照组（8D，12D）心肌Hyp、CVF、AGEs和 RAGE与正常对照组（8C，12C）相比均显著升高；2型糖尿病大鼠心肌AGEs/RAGE水平与CVF呈显著正相关。结论 高脂饮食饲喂加腹腔注射小剂量STZ，能够成功复制Ⅱ型糖尿病大鼠模型。随着病程的延长，心肌纤维化和心肌AGEs含量、RAGE蛋白表达均呈逐渐上升趋势，且Ⅱ型糖尿病大鼠心肌AGEs/RAGE水平与心肌纤维化存在有正相关关系。 关键词：高脂饮食；链脲佐菌素；Ⅱ型糖尿病；心肌纤维化；AGEs/RAGE The Gradual Changes of Myocardial Fibrosis and AGEs/RAGE Levels of Diabetic Rats HOU Gai xia1，XIAO Guo qiang2, XI Xue feng1, LIU Qian qian1 （1. School of Physical Education, Henan University, Kaifeng 475001, China；2. School of Physical Education, South China Normal University, Guangzhou 510006, China） 【ABSTRACT】 Objective In order to investigate the gradual changes of myocardial fibrosis and myocardial AGEs/RAGE levels of type Ⅱ diabetes, the authors established the rats model for type Ⅱ diabetes. Methods Male SD rats were 基金项目：河南省教育厅科学技术研究重点项目（项目编号14B890010）。 作者简介：侯改霞（1978-），女，副教授，博士，研究方向运动与常见慢性病防治， Email： HYPERLINK mailto:hgx715@163.com hgx715@163.com。 fed high-fat fodder for 6 weeks, and then they were injected a small dose of streptozotocin (STZ, 30mg/kg) after overnight fasting 12h. The random blood GLU was detected in the 3th and 7th day respectively after STZ injection. When the rats of which the random blood GLU concentration was greater than or equal to 16.7 mmol/L in two times were successful rats’ model of type Ⅱ diabetes. Six rats of group C and six rats of group D with fasting 12h were killed after intraperitoneal anesthesia at the 4th, 8th and 12th week after the type Ⅱ diabetic rats successfully established. The blood was used f