代谢工程发展20年-SemanticScholar.PDFVIP

生 物 工 程 学 报 Chin J Biotech 2009, September 25; 25(9): 1285-1295 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ © 2009 Institute of Microbiology, CAS CSM, All rights reserved 代谢工程与细胞工厂专栏 20 张学礼 (), 300308 : 代谢工程从上世纪90 年代初期发展至今已有20 年历史, 对微生物发酵工业的发展起到了极大的推动作用。以 下回顾了代谢工程发展至今的三个重要阶段, 讨论了各阶段中代谢工程在技术方面的进展及其对微生物发酵工业的促 进作用。最后还讨论了代谢工程将来发展中的关键问题及解决策略。 : 代谢工程, 微生物发酵, 生物基产品, 系统生物技术, 代谢进化, 合成生物学 Twenty years development of metabolic engineering– a review Xueli Zhang Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China Abstract: Metabolic engineering has been developed for nearly 20 years since its beginning on 1990s, and it has significantly promoted the improvement of microbial fermentation industry. This review summarized the technology development and their applications in fermentation industry in each of the three important phases during the development of metabolic engineering. Finally, the key issues for future development and solving strategies were discussed. Keywords: metabolic engineering, microbial fermentation, bio-based products, systems biology, metabolic evolution, synthetic biology 30 , , , (DNA ) ; , , : , ()