组蛋白甲基化.PPT

Bisulfite sequencing Bisulfite (HSO3) can switch unmethylated C into U (or microarray analysis) The first comprehensive DNA methylation map of an entire genome ---DNA Methylation in Arabidopsis The met1-1, which is a methyltransferase mutant, homozygote exhibits a delay in flowering time that is accompanied by the production of additional rosette and cauline leaves before flowering stem elongation. DNA methylation: Bisulphite treatment the methylcytosine immunoprecipitation (mCIP) method + whole-genome tiling microarrays were used to map methylated component of the genome; Gene expression: