样品处理方法对皖南葛根和粉葛中总黄酮和葛根素含量的影响.PDFVIP

·78· Chinese Journal of Information on TCM Nov.2017 Vol.24 No.11 样品处理方法对皖南葛根和粉葛中 总黄酮和葛根素含量的影响 常跃兴，郭爱灵，陈铎葆，邓云，张荣波，姚斯琪 安徽理工大学医学院药学系，安徽 淮南 232001 摘要：目的 建立葛根和粉葛中总黄酮和葛根素含量测定方法，比较室温超声法、超声加热法和加热回流 法等样品处理方法对葛根和粉葛中总黄酮和葛根素含量测定结果的影响。方法 总黄酮含量测定采用紫外分光 光度法，在波长 250 nm 处测定吸光度；葛根素含量测定采用 HPLC ，固定相为十八烷基硅烷键合硅胶，流动 相为甲醇-水（25 ∶75 ），检测波长为250 nm ，流速为 1.0 mL/min。结果 室温超声法、加热回流法和超声加 热法葛根中总黄酮含量分别为 15.09%、14.48%、12.71% （n ＝3 ），葛根素含量分别为 4.37% 、4.09% 、3.80% （n ＝3 ）；粉葛中总黄酮含量分别为2.09% 、2.23% 、2.17% （n ＝3 ），葛根素含量分别为0.50%、0.53%、0.52% （n ＝3 ）。结论 室温超声法、超声加热法可替代加热回流法分别用于葛根和粉葛中总黄酮和葛根素含量测定 时供试液制备；皖南野生葛根和粉葛中葛根素含量均符合药典标准。 关键词：供试液制备；葛根；粉葛；总黄酮；葛根素；含量测定 DOI：10.3969/j.issn.1005-5304.2017.11.019 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2017)11-0078-04 Effects of Different Sample Preparation Methods on Total Flavonoids and Puerarin Content from Puerariae Lobatae Radix and Puerariae Thomsonii Radix in South Anhui Province CHANG Yue-xing, GUO Ai-ling, CHEN Duo-bao, DENG Yun, ZHANG Rong-bo, YAO Si-qi (Pharmacy Department of Medical College, Anhui University of Science and Technology, Huainan 232001, China) Abstract: Objective To establish UV spectrophotometry and HPLC methods for content determinations of total flavonoids and puerarin from Puerariae Lobatae Radix and Puerariae Thomsonii Radix; To compare the ultrasonic method at room temperature, conventional refluxing method and ultrasonic method at heating conditions at the aspect of content determinations. Methods The content determinations of total flavonoids was determined by UV spectrophotometry at 250 nm; the content of puerarin was determined by HPLC with octadecylsilane-bonded silica gel as the stationary phase, a mixture of methanol and w