基于直接质谱技术快速判别樟树化学型-植物学专业论文.docxVIP

Ab Abstract PAGE PAGE III ABSTRACT A chemotype is a chemically distinct entity in a plant ， with difference in the composition of chemical components. The chemotypes of the plants are not morphologically enough to classify them. The phenomenon is a kind of biological diversity in plant species. Being a species of Cinnamomum, Lauraceae, Cinnamomum camphora (L.) Presl is of high economic value. C. camphora is a valuable tree disituated in the south of Yangtze River. It can be further divided into different chemotypes according to the main chemical components of essential oil: linalool-type, camphora-type, cineole-type, isoborneol-type, borneol-type, et al. This paper introduced characteristic and value of differencet chemotype C. camphora. We HYPERLINK /summarize summarized the traditional methods to discriminant chemotypes of C. camphora, and describe innovative methods in this study. In this paper, the static headspace gas phase chromatography - mass spectrometry (SHS-GC-MS) was used to identify the volatile constituents in powder samples of C. camphora firstly. Then the surface desorption atmospheric pressure ionization mass spectrometry (DAPCI-MS) was used to detect camphor powder samples and fresh camphor leaves combined with multivariate analysis methods, respectively. The main results are as follows: Total of 25 samples of C. camphor leaves, 5 for each chemotype, were tested by using SHS-GC-MS. 171 volatile components were analyzed qualitatively and quantitatively. We found 8 compounds were in all samples: β-linalool, camphene, β-guaiene, γ-terpinene, α-thujene, 2-ethyl-furan, α-caryophyllene and D-germacrene. Different chemotypes of C. camphora leaves were characterized with different quantities of chemical components. The results of CA and DA: β-guaiene, 2-ethyl-furan, α-caryophyllene, D-germacrene, δ-cadinene, hexanal, and copaene were important discriminating variables to identify the five chemotype, which could be better distinguished by the 12 volatil