川滇地区麻疯树种质资源遗传多样性的AFLP分析.pdf

ABSTRACT ctlrc口SLinn to Limaof Jatropha belongingJatrophaEuphorbiaceae．is to in oilwhich considered Mexico．，nleseedcanbeextracted originate processing biofuel．Itisakindof tree studiesonthis importantoil—bearingspecies．Atpresent，the focus onBiodiesel those mainly toxicology，but pla／at production，phytochemistyand on andmolecularare rare．The10wild biologyrelatively totally genetics populations 200individualsfromSichuan-Yunnanwere the of regioninvestigatedby technique AFLP and onthe differentiation，and analysiscomparedgeneticdiversity,genetic structureofwild ofthe10 contentsandthemain genetic populationsspecies．The resultsofthis wereas study followings： 1．The CTAB fromthe improvedmethodWasusedtoextractDNA young of leaves Jatrophacurca$Linn．Usingagarosegel absorbance andAFLP testedtheDNA extracted spectrophotometeranalysis samples ， ， fromabovemethod．nemethodobtained DNAwhichhasa high good quality strucmral theOD260／OD280value1．8-1．95．SothismethodWasthe integrity,、)l，itll bestand suitableforAFLP perfectly analysis． 2．AUthe wereselected