骨髓间充质干细胞对体外损伤心肌细胞凋亡的影响-江苏大学.pdf

19 3 ( ) V o.l 19 N o. 3 2009 5 Journa l o f J iangsu U n ive rsity( M ed icine Ed ition) M ay 2009 郭俊芳 张赢予 莫亚宏 王 好 周艳芳 张国辉 ( 2 12002) [ ] : ( m esenchym a l stem cells M SC s) ( doxo rub i c in DOX ) : 3 M SCs 3 SD 1. 0 m g / L DOX 4 h DOX DOX + M SCsEL ISA 1( in sulin like g row th facto r 1 IGF1) MTT M SC s DOX caspase9 casp ase3 : 3 M SCs IG F1 M SCs IG F1M SCs DOX ( P 0. 05) DOX + M SCs caspase9 casp ase3 DOX ( P 0. 05) : M SCs [ ] ; ; ; [ ] R 329. 2 8 [] A [ ] 167 1- 7783( 2009) 03- 0225 - 05 Antiapoptotic effects on dam aged cardiomyocytes cocultured with m esenchym al stem cells in vitro G UO Junf ang, ZHAN G Ying y u, M O Yahong, WAN G H ao, ZH OU Yanfang ZHANG G uohui ( D epartm ent of Card iology th e A f filiated P eop le s H osp ital of J iang su U n iv ersity Zhen jiang J iang su 2 12002 Ch ina) [Abstract] Objective: T o investigate the effects of m esenchym al stem cells ( M SCs) on doxorub ic in ( DOX ) in jured cardiom yocy tes apoptosis in vitro on the cond ition o f coculture and the possib le m ech a n ism. M ethods: M esenchym a l stem ce lls( M SCs) w ere iso lated and purified by adherentscreening m ethod then exp anded in vitro. T he th ird passage w as u sed for study. T he prmi ary cu ltured neona tal SD rat card io m yocytesw ere exposed to 1. 0 m g /L D oxorubic in fo r 4 h to establish exp ermi entalm ode l of tox ic cardiom yo