提高农杆菌基因转化率方法的研究①.pdfVIP

第 25 卷第 2 期 西南农业大学学报 Vd .25 , NO.2 2003 年 4 月 Journal of South\\e st Agricultural Unive lSity Ap r .2003 文章编号 :1000 - 2642( 2003) 02 - 0144 - 03 提高农杆菌基因转化率方法的研究① 刘艳军，杨静慧，杨恩芹，刘玉冬，柳树梧 (天津农学院园艺系，天津 300384) 摘要通过番茄转化过程中农杆菌f侵受染浓度 体的处理，石研开究了提高番茄基因转化率的方法。试验结果为·用过夜培养的农杆菌和稀释ti 10 倍的农杆菌侵染外植体 以及用 l 倍马铃薯伤流液和 1 rng/ L 低浓度的植物生长素处理外植体和农杆菌可以明显地提高番茄的转化率;乙酌丁 香酣和番茄组织浸提液对转化率无显著影响番茄子叶不经过预培养比预培养的转化率高。 关键词基因转化，农杆菌，伤流液，激素 中图分类号 :S 641 .201 文献标识码 :A AN INVESTIGATION OF THE APPROACHES TO ENHANCE GENE TRANSFORMATION RATE WITH AGROBACTERIUM UU Yan- jun , YANG Jh弯 hli ， YANG En- qin , UU Yu- dor弯， UUShu- wu (Horticwture Th partrnent , Ti叫in A吕ricwtural College , Tianjin 300384 , China) Ah主tract :In the experirnent rnethods to increase transforrnation rate of genes in tornato were tried by adjusting Agrobacterium consistence and pre cwture tirne and treating Agrobacterium and explants by acetosyringone , plant horrnones and extractive sap frorn plants . The reswts showed that transforrnation rate was enhanced when the explants were soaked in A吕robacteriurn , which had been cwtured overnight , or in its 10- fold diluent or when Agrobacterium and the explants were irnrnersed in the extractive sap frorn the tornato plants and lAA (1 rng/ L) . Ac etosyringone and ex- tractive sap frorn the tornato plants had no significant effect on transforrnation rate . Potato cotyledons without precwture , as explants , showed a higher transforrnation rate than non - pre - cwture cotyledons K句7 响。rds :gene transforrnation; Agrobacterium; extractive sap fror