广西钦州湾红树林抗菌活性菌株筛选及活性化合物的分离鉴定-环境科学与工程专业论文.docxVIP

万方数据 万方数据 关键词：广西红树林细菌；系统发育分析；条件优化；分离纯化；结构 鉴定 Abstract In the sea ——land “interface intertidal mangrove wetland ecosystem due to the natural environment characteristics of the salinization of soil, swamp, organic matter content and high strength of radiation, which has obvious superiority in the research and development of bio active substances. Mangrove ecosystem is not only the treasure trove of microorganisms found new species and genetic resources, but also to discover new drug lead compounds active substance such as treasure of resources. In this study, we used Staphylococcus aureu as indicator strain, 12 antibacterial strains, named 1-2-3, 1-3-1, 2-2, 2-3, 3-4-1, 4-4-1, 5-2, 5-8, 6-4-2, 6-9, 7-3-3 and 8-3-21 were screened out from 476 bacteria strains which were isolated from mangrove of Guang Xi by the method of agar diffusion. Among them, 5-8 the inhibitory intensity maximum. The Bacteriostatic spectrum, growth curves, antibacterial curves and phylogenetic analysis of strain 5-8 were determined.. The results showed that strain 5-8 metabolites had strong inhibitory activity against Fusarium oxysporum, Phytophthora copsici, Verticillium dahlia Thanatephorus cucumeris and Escherichia coli.. Strain 5-8 in cultured 12h entered the logarithmic phase, into the stable growth period in 60H after culture; antimicrobial activity reaches a maximum value at 120h. Development of molecular identification and system analysis shows that, the mangroves the strains 5-8 isolated from the mangroves belonged to genus of Bacteria, Proteobacteria, Gammaproteobacteria and Erwinia On the basis of optimum fermentation condition from single-factor experiment, we used Plackett-Burrnan design to screen the significant factors influencing the antibacterial activity including fermentation temperature, salinity and peptone. Path of steepest ascent was undertaken to approach the optimal region of significant factors, Box-Behnken design and response surface analysis were adopted to further inv