不同专化型棉蚜对寄主的选择及其机制初探-中国农业科学院棉花研究所.PDF

棉花 学报 Cotton Science 20 17, 29(3): 292―300 不同专化型 蚜对寄主的选择及其机制初探 王丽袁张帅袁雒珺瑜袁王春义袁吕丽敏袁张利娟袁朱香镇袁李春花袁崔金杰* ( 中国农业科学院棉花研究所/ 棉花生物学国家重点实验室袁河南安阳455000 冤 摘要院揖目的铱研究棉花型棉蚜和黄瓜型棉蚜依靠味觉的寄主选择行为及其机制遥 揖方法铱采用培养皿选择法探 究2 种专化型棉蚜的寄主选择行为曰采用气相色谱- 飞行时间质谱联用仪(GC-TOFMS)对棉花尧黄瓜及西葫芦 苗期叶片的次生化合物组分进行测定袁并筛选其差异次生化合物遥揖结果铱行为选择结果显示院棉花型棉蚜和黄 瓜型棉蚜显著性地选择其原生寄主棉花和黄瓜袁2 种专化型棉蚜对西葫芦的选择无显著差异遥 次生化合物测 定结果显示院棉花尧黄瓜及西葫芦苗期叶片中次生化合物种类及含量存在较大差异遥 其中袁棉花叶片中的琥珀 酸和腐胺的相对含量分别为0.8006 和0.1960袁黄瓜叶片中对应的含量分别为0.2409 和0.4401 遥 揖结论铱棉蚜对 寄主的选择经历了初始的嗅觉选择袁完成了味觉的识别袁能够反映出寄主次生化合物对棉蚜寄主选择的影响袁 推测次生化合物种类及含量的差异可能影响不同专化型棉蚜的寄主选择遥 关键词院棉蚜曰寄主专化型曰寄主选择曰次生化合物 中图分类号院S435.622 文献标志码院A 文章编号院1002-7807 2017 冤03-0292-09 院10.11963/ 1002-7807.wlcjj Wang Li, Zhang Shuai, Luo Junyu, Wang Chunyi, L俟 Limin, Zhang Lijuan, Zhu Xiangzhen, Li Chunhua, Cui Jinj ie* ( 455000, ) [Objective] The obj ective of this research is to study the host choice behavior of cotton and cucumber biotype aphid de- pending on taste and the choice mechanism. [Method] Host choice experiments of the two host biotypes were conducted by the method of Petri dish. The metabolites of cotton, cucumber and zucchini leaf in the seedling stage were measured using gas chro- matography/time-of-flight mass spectrometry (GC-TOFMS) and the differential secondary compounds were selected. [Result] The experiments of behavior choice showed that the two host biotypes had a significantly higher choice to their native host, re- spectively, and there was no significant difference to zucchini host. There was a big difference in the content of secondary com- pounds among cotton, cucumber and zucchini. The relative contents of succinic acid and putrescine were 0.8006 and 0.2409 in the leaves of cotton, and 0.1960 and 0.440 1 in cucumber, respectively. [Conclusion] The host choice of cotton aphid experienced the initial olfactory re