菜豆黄花叶病毒中国和叙利亚蚕豆分离物外壳蛋白的序列分析 英文 .pdfVIP

ACTA PHYTOPATHOLOG ICA SIN ICA 37( 4): 368-376(2007) Sequence Analys is o f the Co at Pro te in Gene o f Bean yellow m o sa ic v irus Iso lates from Faba bean in Yunnan, Ch ina and Syria 1, 2 1* 2 Q I Peng , WANG X iao-m ing , HE Yue-qiu 1 ( The N ationa lKey Facility for Crop G ene Resources and Genetic Im provem en,t NFCRI, Institute o f Crop Science, Chinese A cadem y o fA gricultural Sciences, Beijing 100081, China; 2 P lant Pro tection Co llege, Y unnan Ag riculture University, K unm ing 6 0201, China) Ab stract: V iral d iseases are one of them ajor factors for yield loss of faba bean inY unnan prov ince, China. Bean yellow mosaic viru s ( BYMV ) w as identified in faba bean sam ples by T issue Blot Imm unoassay (TB IA ). A ccording to BYMV sequence subm itted to G enBank, a pair of prmi er w as designed and used to amp lify the coat protein (CP ) gene of BYMV in six faba bean isolates, five from Y unnan province and one from Syria, by reverse transcription PCR ( RT-PCR). The am plified fragment w as cloned and in w hich 822 nucleotidesw as forCP gene. TheCP gene w as found to be 86. 4% to 100. 0% and 96. 7% to 100. 0% ho- mo logous among six BYMV isolates at both the nucleotide and am ino acid leve.l The CP gene of six se- quenced iso lates show ed 79. 1% to 97. 9% and 83. % to 98. % nucleotide and am ino acid identitiesw ith 34 BYMV isolates in G enBank. The com parison data of CP gene sequences show ed that BYMV-Ch inese faba bean isolatesw ere closely related to the BYMV-Japanese faba bean iso lates. The NAG motif, a basic structure for aph id transm ission of the virus located at theN-term inus ofCP gene in BYMV, w as presented in all of six iso lates. Key w o rd s: Be