硅掺杂改性羟基磷灰石对大鼠骨髓间充质干细胞增殖分化作用分析-生物材料与组织工程专业论文.docxVIP

华 华 中 科 技 大 学 硕 士 学 位 论 文 II II Abstract Hydroxyapatite has excellent biocompatibility and bioactivity because it is the main inorganic component of human bone tissue. Silicon is one of essential trace element in natural bone tissue which plays an vital role in bone differentiation and formation, So silicon is doped into pure HA to promote osteogenesis. Hydroxyapatite with different silicone concentration was synthesized with chemical precipitation method. Crystal structure, element composition, functional group determination, surface morphology and crystal morphology were detected by XRD, XRF, FTIR, SEM, and TEM. WST and SEM were used to determine proliferation of cells on Si-HA. RT-PCR and Western-Blot were conducted to measure protein expression in mRNA and protein level to evaluat effect of Si-HA on bMSCs differentiation. XRD results show that crystal phase of HA is not affected when silicon concentration is below 2%. FTIR results reveal HA with different silicone concentration were fabricated that Silicone was integrated into the crystal in the form of silicate. Particle size of Si-HA decreased with the increase of Si concentration by SEM.Si-HA was observed in shape of needle by TEM. RT-PCR and Western-Blot show that doping of silicone increase expression of ALP.COLI.OC.P4H protein and promote the process of osteogenesis. Key Word: Hydroxyapatite; doping silicone; Bone-marrow Mesenchymal Stem Cell; Osteocalcin; Prolyl-4-hydroxylase PAGE IV PAGE IV 目 录 摘 要 I AbstractII 1 绪论 1.1 羟基磷灰石材料简介 (1) 1.2 羟基磷灰石改性研究 (5) 1.3 含硅羟基磷灰石研究进展 (7) 1.4 本课题主要研究内容 (9) 2 含硅羟基磷灰石的制备和表征 2.1 前言 (10) 2.2 材料与仪器 (11) 2.3 实验方法 (12) 2.4 结果分析 (15) 2.5 本章小结 (27) 3 含硅羟基磷灰石生物学效应研究 3.1 前言 (28) 3.2 材料与仪器 (31) 3.3 实验方法 (32) 3.4 结果分析 (39) 3.5 本章小结 (45) 4 全文总结 4.1 结论 (46) 4.2 创新点 (47) 4.3 问题与展望 (47) 致 谢 (49) 参考文献 (50) 附录一 攻读学位期间发表的论文目录 (55) 附录二 英文缩写词表 (56) PAGE 10 PAGE 10 第一章 绪论 1.1 羟基磷灰石材料简介 生物材料是指与生物体紧密结合，修复或替换损伤组织或加强其功能的一类材料 [1]。生物材料既可以是天然的，也可以是人工合成的，或者是二者的结