海洋卡盾藻（香港株）批培养技术及其溶血毒素分离纯化-水生生物学专业论文.docxVIP

暨南大学硕士学位论文 暨南大学硕士学位论文 II II 分别棕榈酸(Z-3)，棕榈油酸(Z-4)，十四烷酸（Z-6）3 种脂肪酸类和谷甾醇（Z-1）。 （7）对溶血毒素提取方法进行研究，并测其溶血活性，可知分析缓冲液提取的 溶血活性较高，其次为甲醇提取，第三为水提醇沉法，最后为透析法提取。 关键词：海洋卡盾藻（香港株） 起始密度 营养盐 溶血活性 化合物结构 III III Abstract Hemolytic toxin is produced by ichthyotoxic algae, which is considered to be one of the major causes of fish mortality . Blooms of ichthyotoxic algae have brought huge losses of fish farming. The poisoning mechanism and chemical structure of the hemolytic toxin are still unclear. In this paper, batch culture of Chattonella marina (Hongkong strain) was studied under room condition. Separation and purification of hemolytic toxin from the alga cell were also studied. The results are as follows. The maximum cell density in C. marina cell cycle was studied when the cells were incubated in different incubation-culture medium volume ratio of 1%, 10%, 15%, 20%, 25% and 50%. Except the initial incubation volume of 50%, cell incubated in incubation volume of 1% to 50% all get to the maximum cell density on the 19th day of incubation. Considering various factors, indoor batch culture C. marina cell choose ratio of 15% to 25% are optimal for the gowth of the cell can achieve better results. In the time of adding medium experiment, adding algal culture media on day 12th and 14th， the maximum cell density of C. marina can reached 1.11 × 104cells/mL and 1.31 × 104cells/mL，respectively. Therefor, it is better to add media in the first 14 days. When adding update rate of 20%, 30%, 40% culture medium, cell density of C. marina increased as the ratio increased. However, when the culture medium update rate was 50%, the cell density decreased. The culture medium update rate of 40% benefit the growth of C. marina with the highest cell density of 1.17 × 104cells/mL. So adding update rate of 40% culture medium will be get higher cell density. The levels of nitrate, ammonium, phosphate and silicate in the secondary utilizated culture water were higher than fresh sea water except nitrite. There wa