基于微生物的可控生物制造-微生物学报.PDF

生 物 工 程 学 报 Chin J Biotech 2009, June 25; 25(6): 909-913 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ © 2009 Institute of Microbiology, CAS CSM, All rights reserved 工业生物技术 史续典, 王刚, 王大明, 余龙江, 杨光 , 430074 : 微生物种类丰富, 尺寸涵盖纳米与微米级, 是天然的可用于纳米、微米及多层次跨尺度加工的“基本单元”。 目前的生物制造方法大多不适用于微生物活细胞, 无法发挥其整体的生物学功能及优势。本研究探索并建立了微流控和 磁控的可用于微生物活体的微纳米生物制造新方法, 定位操纵和有序排列微生物活体。以微生物为微纳米机器人, 诱发 其特有的生物学功能, 进行受控自组装等生物制造过程, 由此有望设计和创制一系列新型特殊功能材料和器件。 : 微纳生物制造, 微流控技术, 磁控微生物, 细菌纤维素, 仿生矿化 Controllable bio-fabrication based on microbes Xudian Shi, Gang Wang, Daming Wang, Longjiang Yu, and Guang Yang National Engineering Research Center for Nano-Medicine, College of Life Science Technology, Huazhong University of Science Technology Wuhan 430074, China Abstract: Microorganisms in nature have rich variety, whose sizes are from nano scale to micro scale. Therefore, microbes can be used as natural “building blocks” in nano/micro multi-level fabrication processes. At present, most of the bio-manufacturing methods do not apply to direct control of living microbes. Their microbiological global functions and superiorities are not available. In this paper, two novel nano/micro bio-fabrication approaches, micro-fluidic control method and magnetic control method have been established. The living microbes could be manipulated to form micro-scaled patterns or to move orientedly. By these approaches, living microbes are taken as nano/micro robots. We could employ their specific biological functions and regulate their controllable self-assembly, which is expected to de