磁化水辅助磷脂酶对大豆油脱胶工艺研究.pdfVIP

2017 年第 30 卷第 3 期 粮食与油脂 81 磁化水辅助磷脂酶对大豆油脱胶工艺研究 付　磊，马　楠，刘雪娇，鹿保鑫 （黑龙江八一农垦大学食品学院，　黑龙江大庆　163319 ） 摘　要：比较磁化水辅助磷脂酶Lecitase Ultra 和去离子水辅助磷脂酶Lecitase Ultra 对大豆油脱 胶率的影响。在单因素试验基础上，利用Plackett–Burman 试验设计筛选出对脱胶率影响显著的 因素，并利用Box–Benhnken 试验设计优化磁化水辅助磷脂酶对大豆油脱胶工艺。最佳脱胶工艺 条件为磁化水添加量3%、酶添加量34 mg/kg、反应时间3.2 h 、反应温度46 ℃、pH 4.8，大豆油的 脱胶率可达95.03%，在同等条件下，磁化水比去离子水脱胶率提高了4.54%。磁化水辅助磷脂酶 Lecitase Ultra 可以有效降低大豆油中磷脂含量，为工业生产提供有力的参考依据。 关键词：磷脂酶Lecitase Ultra ；大豆油；磁化水 Research of degumming process of soybean oil with magnetized water assisted phospholipase FU Lei ，MA Nan ，LIU Xue-jiao ，LU Bao-xin （College of Food Science ，Heilongjiang Bayi Agricultural University ，Daqing 163319 ， Heilongjiang ，China ） Abstract ：The effects of magnetized water assisted phospholipase Lecitase Ultra and deionized water assisted phospholipase Lecitase Ultra on the degumming rate were compared. Plackett–Burman experimental design was used to screen the significant factors on degumming rate based on the single factor experiment. Box–Benhnken experimental was designed to optimize the soybean oil degumming technology of the magnetized water assisted phospholipase. The optimal conditions for the degumming process were as follows ：The dosage of magnetized water was 3% ，the amount of enzyme was 34 mg/kg ，the reaction time was 3.2 h ，temperature was 46 ℃and pH 4.8. Compared to degumming rate of deionized water ，the degumming rate of the magnetized water assisted phospholipase reached to 95.03%，increased by 4.54% under the same conditions. Magnetized water assisted phospholipase Lecitase Ultra could effectively reduce the content of phospholipids in soybean oil ，and provided powerful references for in