野生与养殖全蝎酶解后主要成分含量及抗肿瘤作用的对比研究.pdf

中南药学2018 年 2 月第 16 卷 第2 期　Central South Pharmacy. February 2018 , Vol. 16 No.2 野生与养殖全蝎酶解后主要成分含量及抗肿瘤作用的对比研究 1 2 1 1 1* （山东中医药大学药学院，济南　250355） 桑晓 ，张瑞，王彦多，毛会秀，史磊 摘要：目的　观察比较野生与养殖全蝎酶解后主要成分变化及抗肿瘤作用效果。方法　分别采用改良 Lowrry 法测定蛋白质含量；阿利新蓝法测定糖胺聚糖含量；半微量凯氏定氮测定水解度；MTT 法测定 对A549 和Hep-2 细胞的抑制率。结果　对所得含量用t- 检验：双样本等方差假设分析所得实验数据， 比较两者间差异，其中蛋白质含量、糖胺聚糖含量差异均无统计学意义；野生全蝎的水解度为16.87%， 养殖全蝎为16.46%；两者对A549 细胞和Hep-2 细胞的抑制作用均成剂量关系，随药物浓度的升高抑 制率呈现上升趋势，且养殖全蝎酶解后其抑制效果总体优于野生全蝎酶解后的效果。结论　经过两者间 几个主要成分含量的比较以及对肿瘤抑制作用的比较，野生与养殖全蝎酶解后主要化学成分含量变化不 大，但养殖全蝎酶解后肿瘤细胞的抑制作用优于野生全蝎。 关键词：野生全蝎；养殖全蝎；蛋白质；糖胺聚糖；水解度；抗肿瘤 中图分类号：R285.5　　　　文献标识码：A　　　　文章编号：1672-2981(2018)02-0171-04 doi:10.7539/j.issn.1672-2981.2018.02.007 Content of main components of the wild and breeding scorpion after enzymolysis and comparison of antitumor activity 1 2 1 1 1* SANG Xiao , ZHANG Rui , WANG Yan-duo , MAO Hui-xiu , SHI Lei ( College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355) Abstract: Objective To observe the content of main components of wild and breeding scorpion after enzymolysis and compare their antitumor activity. Methods Protein content was determined by modified Lowrry method and glycosaminoglycan content was determined by the Alcian blue method. The degree of hydrolysis was determined by semi micro Kjeldahl method, and the inhibition rate of A549 and Hep-2 cells was determined by MTT. Results t-test was used to compare the difference between the protein contents by assuming and analyzing the experiment data with the variance of a double sample. The protein contents and glycosaminoglycan contents had no significant difference. The degree of hydrolysis of wild scorpion was 16.87%, whil