土壤霉菌菌丝球的筛选鉴定及固定化菌丝球的应用.PDFVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2019, 35 1 :76-81 土壤霉菌菌丝球的筛选、鉴定及固定化菌丝球的应用 林胜红 潘晓梅 石晓玲 Ihsan Ali 刘震飞 于爽 张锦锋 牛振峰 田永强 （兰州交通大学，兰州 730070） 摘 要 ： 从土壤中分离纯化真菌并鉴定为烟曲霉 L-3。 以菌株 L-3 作为固定化载体 ，将地衣芽孢杆菌固定在真菌上组成固定 化体系。研究了混菌菌丝球 ，菌丝饼，发酵混合液 ，粗漆酶液对刚果红染料废水的降解情况并对染料废水进行毒性试验。结果表明 ， 菌丝球对染料废水的降解效果最显著，降解率高达 99.96%，菌丝饼仅用 20 s 降解率为 91%，发酵混合液与粗漆酶液的处理效果并 不显著。该体系对染料废水的去毒率较明显 ，尤其是菌丝球的去毒率可达到 78%。可见 ，固定化体系对染料废水不但有较高的降 解能力 ，也有较高的去毒率。 关键词 ： 烟曲霉 ；固定化 ；刚果红 ；毒性 ；菌丝球 DOI ：10.13560/ki.biotech.bull.1985.2018-0542 Screening and Identification of Soil Mycelial Pellets and Application of Immobilized Mycelium LIN Sheng-hong PAN Xiao-mei SHI Xiao-ling IHSAN Ali LIU Zhen-fei YU Shuang ZHANG Jin-feng NIU Zhen-feng TIAN Yong-qiang （Lanzhou Jiaotong University ，Lanzhou 730070 ） Abstract: A fungus isolated and purified from soil was identified as Aspergillus fumigatus L-3. Using strain L-3 as immobilized carrier， on which Bacillus Licheniformis was immobilized to form an immobilized system. The degradation of Congo red dye wastewater by the composite mycelial cake，mycelia，fermentation mixture，and crude laccase enzyme solution，as well as the toxicity test of the dye wastewater were conducted. The results showed that the degradation effect on the dye wastewater by mycelial pellets was the most significant，the degradation rate reached 99.96% ；and the degradation rate by mycelia cake was 91% with only 20 s，while the degradation effect by the fermentation mixture and the crude laccase solution wa