玉米谷蛋白抗氧化肽的分离纯化与活性研究.pdfVIP

2018 年第 31 卷第 3 期 粮食与油脂 45 杨 翠，卓婷烨，李祖明，刘 丹 ( 北京联合大学应用文理学院 北京 100191) ， 采用Alcalase 蛋白酶酶解从玉米脱脂蛋白粉中分离得到的玉米谷蛋白 对酶解产物 摘 要： ， 采用超滤 凝胶过滤层析和高效液相色谱法分离纯化玉米谷蛋白抗氧化肽 并研究其抗氧 、 ， 化活性 结果表明 Alcalase 蛋白酶酶解玉米谷蛋白获得的抗氧化肽分子量主要集中在 。 ， 低于 1 kDa ，其DPPH 自由基和羟自由基清除率分别为80.46%、81.36%。凝胶过滤层析得 到抗氧化活性较高的组分P6 、P7 和P8 ，其DPPH 自由基清除率分别为32.05%、24.43% 、 14.19%，多肽含量分别为0.638、0.253、0.158 mg/mL。高效液相色谱对这3 个组分进一步 分离纯化分别得到主要组分。 玉米谷蛋白 抗氧化肽 分离纯化 关键词： ； ； Study on separation and purification of antioxidant peptides from corn glutelin and their activities YANG Cui, ZHUO Ting-ye, LI Zu-ming, LIU Dan (College of Applied Arts and Science, Beijing Union University, Beijing 100191, China) Abstract: The corn glutelin extracted from corn gluten meal was hydrolyzed by alcalase protease. The hydrolysates were separated and purified by ultrafiltration, gel filtration chromatography and high performance liquid chromatography(HPLC) to obtain antioxidant peptides, and their antioxidant activities were studied. The results indicated that the small-sized peptides ( M 1 kDa) which were w the major fractions of the hydrolysates possessed higher antioxidant activities, and their DPPH radical scavenging rate and hydroxyl radical scavenging rate were 80.46%and 81.36%, respectively. P6, P7 and P8 from gel fi ltration chromatography possessed higher antioxidant activities with DPPH radical scavenging rate of 32.05%, 24.43