淡水绿藻在培养基中生长及油脂积累研究.pdfVIP

48 CHINA OILS AND FATS摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 2014 Vol郾39 No郾4 油脂化学 淡水绿藻在培养基中生长及油脂积累研究 张摇 森,刘平怀,杨摇 勋,郝宗娣,张摇 玲,罗摇 宁,吴摇 楠 (海南大学材料与化工学院,热带作物种质资源保护与开发利用教育部重点实验室,海口570228) 摘要:为筛选出最适合进行油脂生产的藻株及其较适生长和油脂积累的培养基,采用柱状光生物反 应器进行一次性培养,对微藻 Tetranephrisbrasiliensis DL12、Ankistrodesmusgracilis CJ09、Ankistrodes鄄 mus sp. CJ02及Desmodesmussubspicatus WC01在4种常用淡水绿藻培养基BG11、3N-BBM、SE及 TAP 中的生长和油脂积累进行研究。 结果表明:3N -BBM 有利于微藻的生长和生物量的积累, BG11有利于微藻细胞内油脂的积累;16组培养试验中,Tetranephrisbrasiliensis DL12在3N-BBM 中 的生物量产率((257.65依45.52) mg/ (L ·d))和油脂产率((80.34 依14.19) mg/ (L ·d))最高, Ankistrodesmusgracilis CJ09在BG11 中的油脂含量(42.72% 依0.60%)最高。 Tetranephrisbrasiliensis DL12 最具油脂生产潜力,其最适生长和油脂积累的培养基为3N-BBM。 关键词:培养基;微藻;生长;油脂积累 中图分类号:TS222;S968.4摇 摇 文献标志码:A摇 摇 文章编号:1003-7969(2014)04 -0048-06 Growth and oil accumulation of microalgae strains isolated from freshwater cultured in mediums ZHANG Sen,LIU Pinghuai,YANG Xun,HAO Zongdi, ZHANG Ling,LUO Ning,WU Nan (Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources,Ministry of Education,College of Materials and Chemical Engineering,Hainan University,Haikou570228,China) Abstract:The batch culture wasconductedby columnphotobioreactor so asto select the microalgae most suitable foroilproductionandtheculturemediumsuitableforgrowthandoilaccumulationofthemicroal鄄 gae. The growth and oil accumulation of four microalgaes(Tetranephrisbrasiliensis DL12,Ankistrodesmus鄄 gracilis CJ09,Ankistrodesmus sp. CJ02 and Desmodesmussubspicatus WC01) in four culture mediums (BG11,3N-BBM,SEandTAP)wereinvestigated. Theresultsshowedthatthe3N-BBMmediumwas benefit for the growth and biomass accumulation of microalgaes;the BG11 medium was beneficial to oil accumulation of microalgaes;in sixteengroupsof