亳芍生物碱提取及含量测定-中国中医药信息杂志.PDF

2013年 10月第 20卷第 10期 中国中医药信息杂志 ·49· 亳芍生物碱提取及含量测定 1,2 1 陈乃东 ,周飞 1.皖西学院生物与制药工程学院,安徽 六安 237012；2.皖西中药与天然药物工程技术研究中心,安徽 六安 237012 摘要：目的 探讨亳芍生物碱提取、定性鉴别及含量测定方法。方法 95%乙醇提取亳芍干粉,总提物回收乙醇 后盐酸酸化,二氯甲烷萃取除杂,加氨水中和至弱碱性后氯仿萃取获总生物碱提物,薄层色谱检测,改良碘化铋钾等显 色,酸碱滴定法测定总生物碱含量。结果 总生物碱提取物经薄层色谱展开后,改良碘化铋钾显色,可检测到4个橙红 色斑点；浓硫酸显色,其中 2 个斑点呈紫红色；甲醛浓硫酸显色,可见 4 个紫黑色斑点；以浓硝酸及茚三酮显色未见 明显色斑。亳芍总生物碱含量约为0.079%。结论 亳芍中含有生物碱,其中可能包含乌头碱类生物碱。 关键词：亳芍；生物碱；显色反应；酸碱滴定 DOI：10.3969/j.issn.1005-5304.2013.10.018 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2013)10-0049-03 Extraction and Determination of Alkaloids from Bozhou Peony Paeonia lactiflora CHEN Nai-dong1,2, ZHOU Fei1 (1.College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Liuan 237012, China； 2.West Anhui Biotechnology Research Center of Chinese Traditional Medicine and Natural Medicine, Liuan 237012, China) Abstract ：Objective To explore the extraction and assaying of the alkaloids from Bozhou Peony Paeonia lactiflora. Methods Dry root powder of the medical material was extracted by 90% ethanol. The extraction was first acidified by hydrochloric acid after recovering alcohol, and then extracted by dichloromethane. The acid solution was adjusted pH to alkalescence by ammonia water, then extracted by chloroform to get crude alkaloid. Bozhou Peony alkaloids were confirmed by TLC along with different colouration method, and its content was detected by acid-base titration. Results Four alkaloid-representative salmon patches were observed by colored with improving potassium heptaiodobismuthate method, and two prunosus blotches, four dark prunosus spots were delected by