Ghrelin基因在PCOS患者卵巢颗粒细胞中的表达下调.DOCVIP

Ghrelin基因在PCOS患者卵巢颗粒细胞中的表达 王炼炼，李 聪，杨 曦，孟江萍，幸贵邦 （重庆医科大学附属第一医院妇产科） 【摘 要】 目的： 探讨Ghrelin基因在卵巢颗粒细胞中的表达及其与多囊卵巢综合征的关系。方法：采用荧光定量PCR及免疫印迹法分别测定11例肥胖型多囊卵巢综合征(PCOS)妇女和36例卵巢功能正常(Non-PCOS)妇女卵巢颗粒细胞中Ghrelin mRNA和蛋白的表达水平。结果： PCOS妇女黄素化颗粒细胞中Ghrelin mRNA的表达和蛋白的表达均低于Non-PCOS妇女(P=0.0149，P=0.0014)。结论：Ghrelin 基因在卵巢中有表达，PCOS妇女卵巢颗粒细胞的Ghrelin mRNA和蛋白表达水平明显低于Non-PCOS妇女，提示该基因可能通过参与了卵泡发育障碍而影响PCOS发生及发展的过程。 【关键词】Ghrelin基因；卵巢颗粒细胞；多囊卵巢综合征 【中国图书分类法分类号】 【文献标志码】 【收稿日期】 Expression of Ghrelin gene in Ovarian granulosa cell with polycystic ovarian syndrome WANG Lian lian, LI Cong，YANG Xi, MENG jiang ping, XING Gui Bang (The Department of Obstetrics and Gynecology, The first Affiliated Hospital of Chongqing Medical University) [Abstract] Objective To determine the impact of the 1evels of Ghrelin in ovarian granulosa cells(GCs) on the pathogenesis invoived in polycystic ovarian syndrome． Methods GCs were obtained during the follicle cells retrieval from a total of 47 wo men：11 women with obesity and type PCOS，and 36 women with functional ovary as the control group. The mRNA expression of Ghrelin protein in GCs. Results The mRNA expression of Ghrelin in GCs was significantly lower in women with obesity and type PCOS than in control(P=0.0149)，and the protein expression of Ghrelin in GCs was also significantly lower in women with obesity and type PCOS than in controls(P=0.0014). Conclusion The mRNA and protein expressions of Ghrelin in GCs were significantly lower in women with obesity and type PCOS than in the control group，which suggests that Ghrelin may be involved in the disturbance of follicular development and influence in the process of the occurrence and development of PCOS. [Key words] Ghrelin gene; Ovarian granulosa cell; Polycystic ovarian syndrome 多囊卵巢综合征（polycystic ovarian syndrome, PCOS) 是临床上常见的妇科生殖内分泌疾病，以肥胖、胰岛素抵抗 ( insulin resistance, IR) 、排卵障碍、雄激素过多为主要临床特点[1]。目前的研究认为，PCOS发病机制之一是卵巢原发性功能障碍[2]。近年研究表明，Ghrelin基因也在卵巢的颗粒细胞中有表达，与颗粒细胞的增殖有关[3,4]。目前已有不少研究探讨Ghrelin基因在肥胖、