冠菌素的发酵分析-茶学专业论文.docxVIP

摘要穗壤豢篡有与聪簿酸、繁麓酸盛粪觳鹣结搦_摹fl劝能，楚攘魏秘生茨调节貔囊。瓣蔫装 摘要 穗壤豢篡有与聪簿酸、繁麓酸盛粪觳鹣结搦_摹fl劝能，楚攘魏秘生茨调节貔囊。瓣蔫装 落黢幕l苯裁羧蕊逶裹瓣生产成本羧裁7=黄在农渡生产上静嶷翔，拯象笈耀发酵法生产二 者的替代物一冠随索+将有利于农业生产。 飙鞠个骰攀骢蕴中筛选得到冠藏素产生菠株003号。紫羚诱变精掰到104号菌株产最 提黼了43．1％，第二敬用N-甲基一N i-硝基州一珏硝基瓤诱窝后褥到212母蘸椿。产鼙谯第一 次蒸黼上疆麓了捷。3％。著鼠褥鞠产蕺莲繁豹掰嘉灏薤拣409号。虽然箕产蘩其存滋黢蔼 株静4{．瓣，毽燕部分抒酸了滠发对冠菝素产受翡隧翻，为今爱的缔造孬|菇溢藏橡鑫《造了 霹裁槛。蓬谯遴纾静第兰次诱燮，希羹辘黟褥剽辩餐撬懿淫豹嚣蘩索产生蘩。 掰难交法霹其发瓣条传遴孝亍德纯。各搿泰妻次鞭痔为：攥魏装鼙，疆、Kx捣、)49SO，· ?珏鼬、NH4CI、攘莓耩。 遥过二次200势发辩壤发辩叟产，获褥豹藏夔索榉晶缝鼹曩饕搜i母片穗绿、抑蒜l丈短 蒸{申长和使舄铃薯块畸形靛生物学活性，经过高教渡相魏谱、毛纲管气谱一质落联阕、核 磁欺撵等检潮，确定为被茹素。 关瓣谶；冠藏豢，缓攀熬蔼，游爱，貔他塔募摹袋俸，簦定 AbstractCoronatine Abstract Coronatine has the similar structure to abscisio acid and jasmonates，They all can regulate the growth of plants．The high cost of production of abascisic acid and jasmonates has limited the application of them+Coronatine,as the substitute of abascisic acid and jasmonates,produced by fermentation，would benefit agriculture．It was confirmed by experiments that No．003，one of Pseudomonas could produce coronatine．After treatment of ultraviolet radiation，a strain No．1 04 with{，43 times than the original strain was obtained．After treatme嫩ofNTG，a strain No．2t2 with 1．14times than No．104 was obtained．On the other side．a strain No．409 which could produce eoronatine in 26(2 Was attained。Although No。409 could only produce 45 percent coronatine ofthe original strain，it had overcome the restriction ofthe high temperature． With one-variable-at-time design。∽optimized fermentation media Was obtained。The optimum media compositions for coronatine fermentation、vem indicated as follows：O．03％KN03O．02％ MgS04’7H20O．1％NH4CI1．5％羽ucose+The optimum coronatine fermentation condition were 50mlbroth／500ml flask,pH7． One gram ofthe crystal ofcoronatine was preparated from the 300 L culture ofNo．104．The crystal could cause the chlorosis of green leaves，hypertrophy of potato tuber discs and could prevent elongation of the stem of SOybe∽．With the result of High performance liquid chromatograph)；Gas chromatography-Mas spectroscopy,Nuclear magnn