基于转录组分析锁阳花序不同发育期花青素合成途径-植物学专业论文.docxVIP

内蒙古大学硕士学位论文Swiss．Prot，KEGG，COG，GO库的Unigenes分别是59547个，46362个，32466 内蒙古大学硕士学位论文 Swiss．Prot，KEGG，COG，GO库的Unigenes分别是59547个，46362个，32466 个，33609个，21040个，39939个，所有注释上的Unigenes是61959个。 3、锁阳花序不同发育期之间(Cy 1 VS Cy一2、Cy-l—VS—Cy-3和Cy 2 VS Cy-3) 进行比对，分别得到8395、21478和17258条差异Unigenes。Cy-1、Cy-2和Cy-3 期上调Unigenes数量逐渐增加，而下调Unigenes数量先增加(从Cy一1到Cy一2)， 随后减少(从Cy．2到Cy．3)。差异基因的GO功能分类和富集分析获得较多的 差异Unigenes涉及代谢通路，KEGG富集差异Unigenes也进一步表明大多数差 异Unigenes参与生物代谢通路。 4、锁阳花序三个发育期差异表达Unigenes中确定参与花青素生物合成途径 的21个Unigenes，通过将这些差异Unigenes与NCBI数据库比对，确定为CHS、 CHI、DFR、LDOX、 (花青素3-0．半乳糖基转移酶)和(UDP．葡萄糖：3-0．糖 基转移酶)，推测这10条基因序列在锁阳花序不同发育期花青素合成途径中起 着关键作用。 关键词：锁阳；测序；通路；差异表达基因 万方数据 内蒙古大学硕士学位论文RESEARCH 内蒙古大学硕士学位论文 RESEARCH 0N ANTHOCYANIN BIOSYN耶HETIC PATHⅥ悛Y OF C鞠＼切?I抑R 2r(，=?MINFLORESCENCE AT DⅡ?FERENT DEVELOPMENTAL STAGES BASED ON TR ANSCRIPTOME ．KR必SIS AB STRACT Cynomorium(Cynomorium songaricum)is herbal medicinal plant that mainly grown in Gansu，Inner Mongolia，Xinjiang and other desert regions．Its root parasitize on the roots of Nitraria which belong to Zygophyllaceae，and Cynomorium is warm-natured，sweet flavour,with the fuctions of tonifying Yang，strengthen digestion．Cynomorium’S main chemical components include terpenoids，flavonoids， lignin，alkaloids and other compounds．Anthocyanins is the most important basic color material of the inflorescence，with the medicinal activity of antioxidant， anti·inflammatory and anti—cancer etc．Reaserch focus on Cynomorium inflorescence transcriptome reveals anthocyanin synthesis pathway,starts new studies for Cynomorium in molecular level，provides a theoretical basis for Mongolian medicine which utilizesstem and inflorescences of Cynomorium and determine the best harvest time of Cynomorium． In this study，a new generation of high-throughput sequencing technology platform Illumina Hiseq2000 is used to do transcriptome sequencing for Cynomorium 万方数据 内蒙古大学硕士学位论文inflorescence 内蒙古大学硕士学位论文 inflorescence in three developmental stages(Cy-1，Cy一2 and Cy一3)，and to analysis the differential exp