微生物耐受乙醇与丁醇机制及其在生物燃料生产与生物转化中.PDF

微生物学通报 Sep. 20, 2014, 41(9): 1864−1871 Microbiology China © 2014 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.130859 专论与综述 微生物耐受乙醇与丁醇机制及其在生物燃料生产 与生物转化中的应用 1,2 3 1 1 1 1 1* 李汉广 周秋香 罗玮 王强 李国莹 赵张敏 余晓斌 (1. 214122) (2. 330045) (3. 330045) 摘 要生物法获取乙醇与丁醇过程中有机溶剂的毒性是生产菌重要环境胁迫因素之一，且当 有机溶剂超过一定浓度时便会抑制微生物的生长，甚至引起微生物的死亡，因此提高工业微生 物的有机溶剂耐受性对工业生产具有重要的意义。对微生物乙醇及丁醇耐受机制的研究可为选 育具有较强溶剂耐受菌提供理论基础。本文系统介绍了微生物耐受乙醇与丁醇的机制，并对其 在生物燃料生产及生物转化中面临的机遇与挑战等问题进行简要的评述。 关键词溶剂耐受性，机制，应用，生物燃料 Applications of microbial ethanol and butanol tolerance in biofuel production and biotransformation LI Han-Guang1,2 ZHOU Qiu-Xiang3 LUO Wei 1 WANG Qiang 1 LI Guo-Ying 1 ZHAO Zhang-Min1 YU Xiao-Bin 1 (1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China) (2. College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China) (3. Affiliated Hospital of Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China) Abstract: Solvent toxicity to producing microorganisms is one of the key stress elements during ethanol and butanol production by microbial fermentation. Furthermore, ethanol and butanol inhibit microbial growth and even cause cell death when the concentration reaches a certain value. Improvement of solvent tolerance of these microorganisms is important for ind