加味金锁二仙饮对雄性小鼠肾精不足证治疗作用及其机理的研究-中西医结合临床专业论文.docxVIP

兰州人学硕土．学位论文ABSTRACT 兰州人学硕土．学位论文 ABSTRACT objective：Established the animal model of kidney essence insufficiency,and observed the growth，development，serunl hormone levels，endurance，metabolism， antioxidant capacity and pathological changes of the mice，to explore the therapeutic effects and mechanism of Modified Jinsuo Erxian Yin on nourishing kidney essence． Method：1．The mice were divided into 6 groups：normal group，model group，Jingui Shenqi Wan group，Modified Jinsuo Erxian Yin high—dose，middle—dose and low-dose groups．Except normal group，others were established the animal model of kidney essence insufficiency．Starting from the eighth day,model group was given saline， Jingui Shenqi Wan group Was given Jingui Shenqi Wan，and Modified Jinsuo Erxian Yin high-dose，middle-dose and low-dose groups were given different concentration Modified Jinsuo Erxian Yin respectively for 1 4 days．Then observed the general situation of animals，measured weights and organ weights，calculated organ indexes and detected the quantities of testosterone(T)，follicle stimulating hormone(FSH)and luteinizing hormone(LH)in serum．2．The mice were divided into 3 groups：normal group，model group and treatment group．Except normal group，others were established the model of kidney essence insufficiency．Starting from eighth day, model group Was given saline，treatment group Was given Modified Jinsuo Erxian Yin for 1 4 days，then observed the swimming time，the climbing time，hepatic glycogen and blood lactate(BLA)．3．The mice were treated as method 2，then observed the superoxide dismutase(SOD)，malonaldehyde(MDA)and pathological changes of brain and testis． Result：Modified Jinsuo Erxian Yin could obviously improve the symptom of kidney essence insufficiency,increase the weights，organ wei曲ts and the quantities of T FSH，LH effectively,prolong the swimming time and climbing time，increase the II 兰州人学硕士学位论文quantity 兰州人学硕士学位论文 quantity of hepatic glycogen，enhance the activity of SOD，decrease the level of BLA and MDA．Compar