利用雄性不育系及异型保持系配制萝卜三交种研究.docVIP

利用雄性不育系及异型保持系配制萝卜三交种研究 摘要：萝卜三交种对萝卜杂交优势及组合力测定的研宄 目前在国内外还很少开展，试验以4套萝卜核质互作型雄性 不育系及1 0个父本自交系为试验材料，配制三交种及单交 种组合，比较了三交种与单交种在产量、生物学性状、抗逆 性、抗病性及耐抽薹性方面的差异。3年的试验结果表明， 大部分三交种较单交种有抗病、高产的表现，配制的9组试 验组合中，6组三交种产量高于单交种，增幅在2.6%? 52.5%；抗病性方面三交种普遍强于单交种；而耐抽薹性与 亲本特性密切相关。 关键词：萝卜；三交种；雄性不育系；异型保持系 中图分类号：S631. 1，S 6 0 3 . 6文献标识 码：A 文章编号：0439- 8 11420-4205-04 Study on the Formulation of Triple Cross Combination of Raphanus sat i v u s with Ma 1 e Sterile Line and Heterot ypic Maintainer Line ZHANG X i ao —kang，XIONG Q i u—fang， ZHANG X u e — q i ng Abstract： The study on t h e three-way cross combinati on of Raphanus sativus L. in determination of hybrid het erosis and combining abilit y was rarely carried out hom e and abroad. The difference in production, biological c haracters, stress resistanc e , disease resistance and re sistant bolting be tween tri pie cross combination and s ingle cross hybrid combinat ion was studied using four s e t s of geno — cytoplasmic mal e sterile lines and 10 male parent inbred lines of R. s a tivus as the experiment mat erials. After 3 years of e x p e riment， the results indicat e d : Most of the three-way c r oss combinations demons t ra ted better disease resistan c e and higher production w h e n compared with single cro s s hybrid. In the 9 groups of experiment combination for mulated， 6 groups of triple cross combination had highe r production from 2.6% to 52.5 % when compared with single c r o s s hybrid, while in diseas e resistance, three-way cro s s combinations were genera 1 1 y stronger than the singl e cross hybrid, and at the s a me time, the bolting resista nee was closely related wit h parent strain characteri s tics. Key words： Raphanus sativ us L. ； triple cross combinat ion； male sterile line； h e t e rotypic maintainer line 萝卜是中国栽培面积仅次于大白菜［B r a s s i c a campestris L. s s p. pekinen s i s O 1 s s o n］的重要蔬菜作物，但杂种利用率却大 大落后于大白菜、甘蓝、黄瓜、番茄等蔬菜作物，目前萝卜 的常规品种仍然占据着市场的较大份额，其主要原因是杂交 育种手段单一及一些技术障碍存在。现在普遍采用的杂交方 式，一是利用雄性不育系配制一代杂种；二是采用自交不亲 和系配制一代杂种。利用自交