抗冷基因RLK转化玉米自交系H99的研究.docxVIP

PAGE \* MERGEFORMAT PAGE \* MERGEFORMAT II 中文摘要 玉米是当今世界一种重要的粮食、饲料和工业原料作物。利用生物技术创造的玉米新材料与人们的生活息息相关。我国东北地区农业气象灾害中低温胁迫问题还较为严重并有待解决，若在玉米生长季节受到低温冷害则会对玉米质量和产量造成影响。近年来，国内外许多科研工作者都在从事抗冷玉米遗传转化的研究，其中农杆菌介导法在玉米遗传转化体系方面取得了巨大的成果。 玉米抗冷基因ZmRLK是高等植物中一类定位在质膜上的类受体蛋白激酶（receptor-likeprotein kinase，RLK）。基因全长由2028bp核苷酸序列组成，编码675个氨基酸。能参与植物细胞内的蛋白互作，在抗逆防御、胁迫应答、信号感知和传递等过程中发挥重要作用。 本研究以玉米优良自交系H99胚性愈伤组织为受体，通过农杆菌介导法将抗冷基因RLK转入玉米，共侵染800块玉米愈伤组织，经双丙氨膦筛选后进行器官的分化,最终成苗450株，分子检测获得含有Bar基因阳性植株24株，阳性率5.3%，目的基因RLK基因表达阳性植株10株，阳性率2.2%。 本研究利用基因工程将抗逆基因转入玉米优良自交系，减少损害，使后代具有抵抗逆境的能力，从而更深入地研究各种调控因素玉米在生长发育中的影响，培育出适应环境的抗冷玉米新种质，为提高玉米抗冷性能奠定实验基础。 关键词：玉米；抗冷基因RLK；胚性愈伤组织；农杆菌介导法 PAGE \* MERGEFORMAT PAGE \* MERGEFORMAT II Abstract Zea mays is a kind of important grain, feed and industrial raw material crop in the world today. Peoples lives are closely related to the use of biotechnology to create new corn materials. The problem of low temperature stress in agrometeorological disasters in northeastern China is still serious and remains to be solved. During the growing season, if the chilling injury in maize is affected by low temperature , the quality and yield of maize will be affected. In recent years, many scientific research workers at home and abroad are engaged in the transformation of cold maize genetic research, in which Agrobacterium-mediated transformation of maize genetic transformation system has made great achievements. Maize cold-resistant gene ZmRLK is a class of receptor-like protein kinase (RLK) located in the plasma membrane. The full length of the gene consists of 2028bp nucleotide sequence encoding 675 amino acids. The gene can participate in plant cell protein interaction, and plays an important role in the anti-stress defense, stress response, signal perception and transmission process. In this research, we used the H99 embryogenic callus of maize inbred line as the recipient, and the cold resistance gene ZmRLK was transferred into maize by Agrobacterium tumefaciens, and 800 maize c