急性应激后大鼠肠功能紊乱模型中树突状细胞以及协同刺激分子CD80、CD86的表达-内科学(消化系病)专业论文.docxVIP

安徽医科大学硕士学位论文 安徽医科大学硕士学位论文 5 5 ileum and distant colon mucosa in two groups were measured,and immunohistochemistry﹙S-P method﹚were used to detect the expression of intestinal surface DC,constimulatory molecules CD80 ,CD86, and enzyme-linked immunosorbent assay(ELISA) were used to detect the expression of VIP、IL-1β.VIP、 IL-1βin peripheral blood. Results Compared with control group,the stool character of experimental rats had changed from the sixth day, and the sensory threshold ofintestinal were evident decreased(P0.05); There were no significant difference between the two groups through the macroscopic and histological score;The expression of DC,CD80,CD86 in terminal ileum was similar to that of controls（P0.05）;whereas the expression of VIP,IL-1β in terminal ileum was markedly increased compared with control group (P0.05),and positive liner correlation between VIP and IL-1β was observed in terminal ileum（r=0.78,Pα0.01）.In distant colon, the expression of constimulatory molecules CD80,CD86 and VIP was markedly increased compared with control group (P0.05),but the expression of CD11c and IL-1β in distant colon was similar to that of control（s P0.05）. and positive liner correlation between VIP and CD80 was observed （r=0.70, Pα0.01）,the positive liner correlation between VIP and CD86 was observed in distant colon(r=0.34, Pα0.05). The expression of blood VIP、IL-1β were all increased significantly compared with control group (P0.05). Conclusion There have been low grade inflammation in the terminal ileum and distant colon of the rat model with intestinal dysfunction induced by cold-restraint stress. The existence of mild intestinal inflammatory was verifyed by the expression of constimulatory molecules CD80,CD86and DCs from the perspective of immune response. We speculate that CD80,CD86 in distant colon may play an important role in intestinal immune regulation after intestinal dysfunction,and may be the biological indicators of inte