家兔视神经压迫性损伤后视网膜及视神经动态病理改变的实验研究-外科学专业论文.docxVIP

PAGE PAGE 10 中 文 摘 要 和 72h 分别比正常对照组低 0.56% 2.26% 3.39% 4.52% 6.78% 26.55% 30.51% 经检验视神经钳夹损伤后 48h 组与 24h 组有显著性 差异P0.05各对照组之间比较无显著性差异 4 视网膜神经 节细胞在 24h 内出现凋亡 24h~48 小时 RGCs 凋亡速率最快 结论 本试验模型可造成明确的视神经和视网膜损伤 较贴近于 临床视神经管内持续高压状态 视网膜和视神经损伤的严重度与时间 呈相关性 钳夹 24h 就会产生不可逆性损伤 故推测临床视神经损伤 确诊后 如需手术减压应在 24 小时内实施 关键词 视网膜节细胞 视神经 神经髓鞘 凋亡 损伤 英 英 文 摘 要 A Study on the Morphological Changes of Optic Nerve and Retina after Optic Nerve Crush in Rabbits Abstract Objective 1.In order to investigate the pathological changes in optic nerve and retina after strike of optic nerve. 2.Using the staining method of TUNEL to search the time limits of retinal ganglion cells ( RGCs ) irreversible injury, for defining the time limits of the treatment after optic nerve injuries. Materials and method 1 32 rabbits approximate three kilograms were divided into eight groups at random, include one normol group and seven injury groups. There are four rabbits in each group. 2 The rabbits of injury groups were exposed the optic nerve. We used the trauma-free clamp (5mmH2O) to clamp the optic nerve at 3mm away from eyeball. The histopathology was studied with light microscope at 2h, 4h, 8h, 16h, 24h, 48h and 72h after injury. The cell apoptosis of RGCs from the injured retina were examined by the TUNEL reaction. Optic nerve was observed by electric microscope. 3 To study the mechanism of RGCs and optic nerve by comparing all the data obtained according to the methods mentioned above in experiments, which is expressed by meanstandard deviation, sorted by Excel and analyzed by SNK-Q test of SPSS(11.5) statistic package. Result 1.Myelin stain of the optic nerve:normal groups: Myelin is intact and distributed evenly. It was dyed blue-black.Injury groups: 2~8 hours after the optic nerve injuries, the glia cell had proliferated; In 16 hours ,a large quantity of vacuoles were between nerve fiber; In 24 hours, myelin swelled and demyelination appeared; In 48 hours, the optic nerve began to degenerate and infarct