寄生通络巴布膏的研制-中药学专业论文.docxVIP

提 提 要 本文对治疗骨痹的中药复方制剂寄生通络巴布膏进行了系统的研究。采用b(27) 正交试验筛选出鱼鳔的减压提取工艺；采用L。(34)正交试验筛选出桑寄生、丹参、 蜈蚣、威灵仙的50％乙醇提取工艺；独活、细辛水蒸气提取挥发油后，采用L。(34) 正交试验筛选出其水煎煮的提取工艺；依据巴布膏剂及药材特性，确定了乳香、没药、 芥子、人工麝香粉碎入药的工艺。通过粘着力试验确定了鱼鳔作为本品粘附剂；根据 其剥离强度、最后涂布和保湿情况确定丙二醇的用量；采用体外透皮吸收试验对透皮 促进剂的使用进行了研究；确定了本品成型工艺，通过三批样品的研究证明了工艺的 可行性。制定了本制剂质量标准，采用薄层色谱法对处方中独活、丹参、没药进行了 定性鉴别，采用高效液相色谱法对臣药丹参中活性成分丹酚酸B进行了定量控制， 采用气相色谱法测定了贵细药人工麝香中麝香酮的含量。进行了初步稳定性考察、药 效学及安全性试验的初步研究，结果表明该制剂稳定性较好，有明显的抗炎、镇痛作 用。对皮肤安全无毒、无刺激。 关键词寄生通络巴布膏；正交试验；工艺研究；质量标准；药效学 Preparation Preparation and Evaluation of Jisheng Tongbi Babu plaster Spedafity：Pharmacy of TCM Author：HeHui Tutor：L证Gui-tao Systematical research of the compound prescription of TCM has been performed in this study．Jishcng Tongbi Babu plaster is used to treat Osteoalthristis．By orthogonal test,the extraction technology of Yubiao，Herba TaXilli，Radix Salviae miltiorrhizae，Radix Clematidis and Scolopendra is screened．the extraction technology of Radix Angelicae pubescentis and Herb Asari after volatile Oil ofthem iS obtained．Olibanum．Myrrha,Semen Sinapis and Moschus Artifactus grinded．The optimum proportion ofmatrix proportion for the preparation process of Jisheng Tongbi plaster is selected by test．A check·up for adlfibition,nonflowing,content of cream is founded．The result shows Jisheng Tongbi Babu plaster which is made bY preparation condition of this test accords witll concemed regulations of Babu plaster of CERadix Angelicae pubescentis,Radix Salviae miltiorrhizae and Myrrha qualitatively tested by TLC．Salvianolic acid B and Muscone is quantitatively analyzed by HPLC and GC．Primary stability and phalmacodynamics ale researched，which shows that Jisheng Tongbi Babu plaster has good stability,it Call obviously ease pain,resist inflammation and be safe to skin without stimulation． Key words Jisheng Tongbi Babu plaster；orthogonal test：technological study： quality standard；phalmacodynamics 原创性声明本人郑重声明：所呈交的学位论文，是在导师的指导下独立完成 原创性声明 本人郑重声明：所呈交的学位论文，是在导师的指导下独立完成 的，文中除注明引用的内容外，不包含任何其他已经发表的科研成果。 对本文研究做出重要贡献者，