几种榕小蜂的分子学鉴定研究-动物学专业论文.docxVIP

Abstract一_—————-————————————————_————一i Abstract 一_—————-————————————————_————一i Abstract In this paper,ribosome 28S，mitochondrion cytodu．ome b(Cytb)，cytocIⅡ．ome oxidase I(COI)and sequence--related amplified polymorphism(SRAP)were used to analysis the phylogenetic relationships，gene sequence，and intraspecific distance among F／c“s p“，卵il口 ＆ Ficus awkeotsang pollinating wasps(1E pumilae)，Ficus virens var． s“b／aneeolata(Miq．)Comer pollinating wasps(Platyscapa coronata)，unpollinatlng _II wasps(CamarothDtax bimasculinus),Ficus altissima pollinating wasps(Eupristina altissima)．The results were as follows： 1．Ribosome 28S analysis result：Ribosome 28S fragments were amplified via polymerase thain reaction、recycled and purificated，the sequences analysis as followed： @the average content of A，ljI T，C Was 22．2％，30．08％，22．5 1％，25．20％，the average content of Gc was 55．28％higher than AT；②the Kimura2-paramter distance(K2P) 二 calculated by MEGA3．1，the average distance between the Eawkeotsang pollinating wasps(阡：p“，”ilae)Was 0．0 1 6 1，the average distance between the E pumilae pollinating waLsps(1,K．p“mi／aP)was 0．0103，the average distance between the two group Was 0．0532， the genetic distance between two different morphology C．bimasculinus male wasps of was 0．0069；③the clustering analysis with the method of NJ and UPGMA·The results were aLs foilow：the variation between the W．pumilae via F．pumila and W．pumilae via E口wkeDts口ng mainly came from the inter-group，the genetic relationship between the two different C bi，竹以sc“linus male wasps Was close，belong to male polymorphism·The analysis of NJ and UPGMA proved the above conclusion· 2．The analysis results of Mitochondrion c”ochrome b(Cytb)and c”ochrome oxidase I(COI)：(1)Cytb sequences analysis as followed：①the average content of A， Q T，C was 32．9％，11．2％，41．9％，14．1％，the average content ofGC Was 25．3％lower than AT；G-base Was lower than the others and generally bowed a lack of G-base； ②gene sequence analysis bowed that conserved s