产油脂微藻的分离鉴定及培养条件优化-应用与环境生物学报.PDF

2011 17 6): 897~900 2011-12-25 应用与环境生物学报 ， ( Chin J Appl Environ Biol=ISSN 1006-687X DOI: 10.3724/SP.J.1145.2011.00897 * 产油脂微藻的分离鉴定及培养条件优化 ** 于荣清 刘 义 田思琪 杨云喜 李 伟 曹 毅 乔代蓉 （四川大学生命科学学院微生物与代谢工程四川省重点实验室 成都 610065） 摘 要 为促进自养高产油微藻的工业化应用，从青海多种生境中分离到34株产油微藻，以筛选获得产油脂最高的青 3-2-2株为出发株，18S rRNA分析表明其与栅列藻属（Scenedesmus sp.）同源性达到99%以上，确定该株属于栅列藻属. 研究氮源、培养时间、培养温度、初始pH值等培养条件对微藻生物量及油脂含量的影响，优化培养条件为：硝酸钠为氮 源，以10% （V/ V）接种量接种于普通SE培养基，培养温度为25 ℃，初始pH 7.0 ，培养周期为20 d ，可以获得较高的油脂产率， 比优化前的产率增加了近70%. 图3 表1 参16 关键词 微藻；油脂；栅列藻属；菌株分离鉴定；培养条件优化 CLC Q949.206 : TK6 Isolation and Identification of Oil Microalgae and Optimization of Its Culture Conditions* YU Rongqing, LIU Yi, TIAN Siqi, YANG Yunxi, LI Wei, CAO Yi QIAO Dairong** (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, China) Abstract Thirty-four algae strains were isolated from diverse environments in Qinghai, China. All of them were identified as oil microalgae, and the most oil-rich strain named Qing 3-2-2 was used as the test strain. The homology of 18S rRNA fragments between Qing 3-2-2 and the strains in Scenedesmus genus was high, up to 99% identical. Thus, the strain was identified to belong to Scenedesmus genus. Effects of culture factors, such as N source, incubation time, culture temperature and initial pH