芦笋离体快速繁殖中嫩茎增殖培养对根分化的影响-华北农学报.PDF

华北农学报# 2006, 21( 增刊) : 46- 49 1 2 1 1 沈火林 , 赵 娜, 韩清霞, 戴凡炜 ( 1. , 100094; 2. , 100088) : , : ( ) J (MS+ NAA 0.2 mg/ L + BA 0.5 mg/ L) , 2 J (MS+ NAA 0.2 mg/ L+ BA 0. 1 mg/ L) J (MS + NAA 0.05 mg/ L+ BA 0. 1 mg/ L) 1 ( 3 9 ) , 1G (MS+ IBA 0.5 mg/ L + BA 0.05 mg/ L + 4 2.0 mg/ L PP333) , 90% , 90% 100% , 100% : ; ; :S68 :A : 1000- 7091(2006) - 0046- 05 Effects of Stem-segment Increased Culture on Rooting Differentiation During Quick Reproduction of Asparagus in vitro 1 2 1 1 SHEN Huo-lin , ZHAO Na , HAN Qing-xia , DAI Fan-wei ( 1. Department of Vegetable, Academy of Agronomy and Biotechnology, China Agriculture University, Beijing 100094, China; 2. Beijing Seed Administration Station, Beijing 100088, China) Abstract:The rooting technique on the formation of asparagus tissue culture system were studied in this experiment, including optimization of hormone combination on rooting cultivation, the effects of the new stem subculture, the frequen- cy of transplanting, optimization of hormone combination on subculture of the new stem and the frequency of transplanting have the great effects on rooting regeneration of the following new stem .The best processes of asparagus in vitro are :Af- ter induced on J2(MS+ NAA 012 mg/L + BA 015 mg/ L) , the new stem need subculture one time on J3(MS+ NAA 012 mg/ L+ BA 011mg/ L) or J (MS + NAA 0105 mg/L+ BA 011mg/ L) , Subculture time have the great effect on the fol-