水稻种子含水量及其对贮藏寿命的影响-中国农业科学.PDF

中国农业科学 2005,38(7):1480-1486 Scientia Agricultura Sinica 水稻种子含水量及其对贮藏寿命的影响 1 1 2 2 张玉兰 ，汪晓峰 ，景新明 ，林 坚 1 2 （北京林业大学生物学院，北京 100083 ； 中国科学院植物研究所，北京 100093 ） 摘要：以粳稻幸实(Oryza sativa L. subsp. japonica cv. Xingshi )，籼稻桂早2号(Oryza sativa L.subsp. indica cv. Guizao 2)水稻种子为试材，研究不同含水量水稻种子在-20℃和室温（25℃）条件下贮藏5年后的活 力变化及其生理生化基础。结果表明，适度超干处理能延长种子的贮藏寿命，种子活力保持与种子含水量和贮藏 温度密切相关。籼稻桂早2 号种子耐干性较强，在含水量降至2.4%时，种子活力与5.8%相比无显著差异，而粳稻 幸实种子活力极显著下降。不同含水量水稻种子抗氧化酶活性、可溶性蛋白和热稳定蛋白含量与种子活力呈正相 关，ABA 含量、磷脂酶活性随含水量降低而升高，说明种子耐干性的获得是多种生理机能共同作用的结果。幸实 种子可溶性蛋白含量大大高于桂早2号，这可能是幸实种子较不耐干的关键因素。 关键词：水稻；种子；含水量；贮藏寿命；生理生化 The Effect of Moisture Content on Storage Life of Rice Seeds 1 2 2 ZHANG Yu-lan, WANG Xiao-feng , JING Xin-ming , LIN Jian 1 ( Depatment of Biology, Beijing Forestry University, Beijing 100083; 2Institute of Botany, Chinese Academy of Sciences, Beijing 100093) Abstract: Japonica rice (Xingshi) and indica rice (Guizao 2) seeds were used to investigate the vigor and some physiological indexes after stored at -20℃ and ambient temperature （25 ℃）for five years. The results showed that moderate drying could prolong the storage life of rice seeds. The indica rice (Guizao 2) seeds had strong ultra-dring tolerance. When moisture content fell to 2.4%, the vigor index of Guizao 2 seeds, compared with that of 5.8%, had no obvious change. On the contrary, the vigor index of Xingshi seeds decreased rapidly. The study also indicated that there were positive correlations between the antioxidation s