肌红蛋白和血红蛋白的氧化还原性质和类酶活性的电化学研究-分析化学专业论文.docxVIP

AbstractThe Abstract The redox reactions and enzyme—like properties of myoglobin and hemoglobin were explored bY the electrochemical theories and methodS．The results were summarized as the following： (1)Myoglobin(Mb)，immobilized on glassy carbon electrode(GCE)surface by earrageenan，Can transfer electron directly to electrode surface both in aqueous and aqueous—organic mixture solutions．Proteins entrapped in the carrageenan film undergo fast direct transfer-electroareaetions，corresponding to Fe(III)+e_Fe(II)．The E“ale linearly dependent on solution pH(redox Bohr effect)．indicating the electron njansf．er of Fe(HI)／Fe(II)redox couple companied with the transfer of proton．Ultraviolet visible (UV-Vis)spectra suggest tlmtproteins keep their original conformation in the carrageenan films．and theconformation changes reversibly in a range of pH 3．0-11．0． The mechanisms for catalytic reduction of oxygen，peroxides，and dehalogenation of haloethanes，by proteins entrapped in carrageenan were also explored． (2)Hemoglobin Was entrapped in thecalrageenan film undergo fast direct transfer—electron reactions in aqueous·organi solvents mixtures．The Eo‘ale linearly dependent on solution pH(redox Bohr effect)，indicating the electron transfer of Fe(III)／Fe(II)redox couplecompanied witll the transfer of proton．The mechanisms for catalytic reduction of oxygen，hperoxides，and dehalogenation of haloethanes by hemo掣obin entrapped in carrageenan hydrogel were also explored． Keywords：Myoglobin，hemo#oMn，redox，enzyme-like activity,electrochemistry II 华中师范大学学位论文原创性声明和使用授权说明原刮性声明 华中师范大学学位论文原创性声明和使用授权说明 原刮性声明 本人郑重声明：所呈交的学位论文，是本人在导师指导下，独立进行研究工作 所取得的研究成果。除文中已经标明引用的内容外，本论文不包含任何其他个人或 集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均己在 文中以明确方式标明。本声明的法律结果由本人承担。 作者签名：勤闩搬 日期：砷年岁月上6日 学位论文版权使用授权书 本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权 保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借 阅。本人授权华中师范大学可以将本学位论文的全部或部分内容编入有关数据库进 行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。同时授权 中国科学技术信息研究所将本学位论文收录到《中国学位论文全文数据库》，并通 过网络向社会公众提供信息服务。 日期：跏1年岁月柏日 孚目 师期 蛩 ，百 蹩 作者签名：勤诗每支