灰毡毛忍冬与正品金银花化学成分的比较研究-中药学专业论文.docxVIP

冬与正品金银花的紫外峰形基本一致，提示其化学成分基本相似。3．高效液相色谱法(HPLC)对样品液中绿原酸、咖啡酸的含量比 冬与正品金银花的紫外峰形基本一致，提示其化学成分基本相似。 3．高效液相色谱法(HPLC)对样品液中绿原酸、咖啡酸的含量比 较结果为： 1)各样品中绿原酸含量测定结果为：隆回、溆浦、新宁种植基地 产的灰毡毛忍冬中绿原酸百分含量分别为3．9±o．45 37、2．6±0．1408、 4．5±0．01 82，河南、山东金银花种植基地产的密银花、济银花和山东 药材市场购得的济银花中绿原酸的百分含量分别为2．4±0。1913、2．1 ±0．1 684、2．5±0．0506。将结果进行比较得知，湖南产的灰毡毛忍冬 中绿原酸的含量比正品金银花中绿原酸的含量均要高。 2)各样品中咖啡酸含量测定结果为：隆回、溆浦、新宁产的灰毡 毛忍冬中咖啡酸百分含量分别为0．05 36±0．006 3、0．0424±0．0086、 0．0447±0．0033，河南、山东金银花种植基地产的密银花、济银花和山 东药材市场购得的湃银花中咖啡酸百分含量分别为0．0477±0．002 3、 0．0647±0．0065、0．0454±0．0146。将结果进行比较得知，湖南产的灰 毡毛忍冬咖啡酸的含量与正品金银花中咖啡酸的含量相当。 4．GC-MS对挥发油成分的分析结果表明：灰毡毛忍冬和其它正品金 银花挥发油中均含有有效成分芳樟醇(Linai001)、香叶醇(Gerani01) 和a一松油醇(a-Terpine01)等多种成分。 关键词：金银花；绿原酸；咖啡酸；挥发油；气一质联用法 Comparative Comparative Study on the Chemical Constituents of Lonicereae macrathoides Hands Mazz．and the Certified Honeysuckle Postgraduale：Tong Qiaozhen Advisor：ProfeSSOf Zhou Ribao (Huna College of Traditional Chinese Medicine Changsha 410007) Abstract The medicinal material honeysuckle comes from the dried buds or primary flowers of Lonicerae japonica Thun．，L．hypoglauce Miq．，L． contusa DC．，L．dasytyla Rehd．Which belong to the Capritolialeae family． Honeysuckle iS not only a usual medicine which can clear away heat and toxic material，expel wind and heat，but also an important raw material which can be used in health drinks，food and chemical industry．With the increasing demand at home and abroad，the market is promosing． L．macrathoides Hands Mazz．，also called liked big flower Lonicerae japonica Thun．，is the main merchant variety of honeysuckle in Hunan． Nowadays，there have been wild researches on pharmacognosy，the technology of cultivation，effective chemical constituents，pharmacology and some other aspects of the certified honeysuckle．However，the study on L．macrathoides Hands Mazz．is limited to the simple characteristic description and the detailed transcription of the effective chemical constituents but the related pharmacology is lacked．In order to scientificly evaluate the medical va