环境温度对达乌尔黄鼠冬眠能量消耗的影响及脂肪酸合成基因的阶段性表达-动物学专业论文.docxVIP

II II 碳五烯酸（Eicosapentaenoic Acid，EPA）合成的酶在冬眠阶段表达量显著增 加。暗示冬眠阶段上述不饱和脂肪酸合成增加，这些不饱和脂肪酸增加细 胞膜活动性和血液流动性，有利于冬眠期细胞功能的维持。 关键词：环境温度，冬眠模式，能量消耗，多不饱和脂肪酸合成 PAGE PAGE IV The impact of ambient temperature on energy consumption and the stage expression of gene combined by fatty acid during hibernation in Daurian ground squirrel abstract Temperature has a great impact on hibernation patterns and energy consumption for mammal. The research object is Daurian ground squirrel.During hibernation, the ambient temperature by manual control is respectively 5±2℃，15±2℃，25±2℃. Under the three different ambient temperature, hibernation patterns and energy consumption under winter in Daurian ground squirrels are measured. We determine gene expression of unsaturated fatty acids during hibernation in Daurian ground squirrels and analysis the function meaning. The main results are as follows: Under different ambient temperature, hibernation patterns in Daurian ground squirrels is different. When ambient temperature is 5℃, Daurian ground squirrels are in deep hibernation bouts and hibernation pattern is deep hibernation. At 15℃, 75 percent of Daurian ground squirrels is deep hibernation and 25 percent of them shows short hibernation. At 25℃, 87.5 percent of Daurian ground squirrels is short hibernation and 12.5 percent of them is no hibernation. That indicates hibernation pattern is different under different ambient temperature in Daurian ground squirrels. High temperature reduces the expression of hibernation. When ambient temperature is at 5±2℃, 15±2℃ and 25±2℃, the length of hibernation season in Daurian ground squirrels is respectively 168.33±6.17d, 146.75±11.45d and 88.38±10.39d. The difference is significant（F（2，22）=19.431, P=0.000.01 ） . The length of three consecutive longest hibernation is respectively 27.4±3.3 d，15.3±1.2 d and 3.88±0.58 d, and the difference is significan（t F（2，22）=28.838，P=0.000.01）. These results suggest that the length of hibernation