拮抗放线菌W7的筛选、鉴定与次生代谢产物的分析.pdfVIP

Abstract The actinomycete strains with its high practical value can produce some kinds of antibiotics which are commonly used in the preparation of new pesticide. Because of its special characteristics such as residue-free, pollution-free and regeneration, it becomes the development directions of pesticides and can effectively avoid many environmental problems brought by chemical pesticides. As far as the quantity, quality and variety are concerned, it cannot meet the production requirements.Thus finding new strains becomes a research focus of the study in the field of actinomycete strains. The actinomycete strain resource in Qinghai province is unique for its unique and complex ecological environment, which offers the possibility to separate and screen new kind of strains. The study is separating and screening the antagonistic actinomycetes in rhizosphere soil of Lycium barbarum L. in Qinghai plateau, which has antibacterial activity on various bacteria. Finally we identified the strains and conducted a preliminary study on its fermentation conditions, the active substance . All the findings are just as follows: 1. 90 actinomycetes strains which had different morphologies and colors were isolated. One strain named W7 was selected by the first and the second screening using the method of agar block and cylinder plate method. W7 had antibacterial activity to Staphylococcus aureus, Bacillus licheniformis, Bacillus subtilis , Bacillus cereus, Bacillus pumilus, Aerobacter aerogenes Pseudomonas aeruginosa and so on. It was the strongest antibacterial activity of Staphylococcus aureus bacteriostasis and the bacteriostatic circle diameter was above 20mm. And the title of fermentation liquid was 2.452u/ml by using two-dos