黄原胶的化学改性及其衍生物抗氧化活性研究-应用化学专业论文.docxVIP

上海海 上海海洋大学硕士学位论文 PAGE PAGE 10 XGOSSA-3。衍生物 XGOSSA-3 抗氧化性最弱的原因可能是取代度最大，导 致活性羟基数目最小，抗氧化性能最差。 4、黄原胶寡糖通过与邻苯二甲酸酐酯化反应，得到取代度不同的三种衍 生物, XGOSPA-1、XGOSPA-2 以及 XGOSPA-3。三种黄原胶马来酸酯衍生物 的抗氧化强弱顺序为：XGOSPA-3 XGOSPA-2 XGOSPA-1。结果表明：在 实验范围内，取代度最高的黄原胶邻苯二甲酸酯衍生物抗氧化性能最好。这 一结果的原因是取代基的吸电子作用在黄原胶邻苯二甲酸酯衍生物的抗氧化 性中起主要作用。 5、黄原胶寡糖通过与马来酸酐、邻苯二甲酸酯化反应制备衍生物。通过 筛选，得到两组取代度相近衍生物 XGOSMA-A、XGOSPA-A (取代度约为 0.3) 与 XGOSMA-B、XGGOSPA-B (取代度约为 0.6)。结果表明，抗氧化性能： XGOSPA-AXGOSMA-A；XGOSPA-BXGOSMA-B。取代度相近，黄原胶 邻苯二甲酸酯衍生物具有更好的抗氧化活性。这一结果的原因可能是黄原胶 邻苯二甲 酸 酯 衍生 物中 -COC6H4COO- 基团 与黄原胶 马来酸 酯 衍 生物中 –COCH=CH-COO-基团相比具有更强的吸电子性，使得邻苯二甲酸酯衍生物 具有更好的抗氧化性。 本文研究了黄原胶寡糖及衍生物结构与抗氧化性能之间的联系。拓展了 黄原胶的生物活性研究以及黄原胶抗氧化机理研究。为进一步扩大黄原胶在 生物、医药等领域的应用提供了思路和依据。 关键词： 黄原胶，寡糖，衍生物，抗氧化性能 Chemical modification and antioxidant activity of xanthan and its derivatives ABSTRACT Xanthan gum (XG) is a microbial polysaccharide produced by the Xanthomonas campestris. Xanthan is a natural polysaccharide and an important industrial biopolymer. The side chain and backbone of xanthan form a double helical state which is stabilized by intermolecular and intramolecular hydrogen bonds. This double helical structure results in the poor solubility of xanthan and the limited antioxidant activity. In this paper, water-soluable xanthan oligosaccharides (XGOS) were prepared by degradation of xanthan. Then xanthan oligosaccharides were modified by maleic anhydride, succinic anhydride, and o-phthalic anhydride, respectively. Their structural changes were confirmed by Fourier transform infrared (FTIR) spectra, their molecular weights were determined by gel permeation chromatography (GPC) method. Their pyruvate acid content, reducing sugar content, substituting degrees were measured. In addition, their antioxidant activities were evaluated by the scavenging of superoxide anion (O2·-), hydroxyl radicals (·OH), 1,1-diphenyl-2-picryhrazyl radical (DPPH), hydrogen peroxide (H2O2) and determination of reducing power. The main results are shown as following: Three xanthan oligosaccharides