糖尿病对大鼠牙周炎影响的试验研究-第三军医大学学报.DOC

糖尿病对大鼠实验性牙周炎影响的研究 邓婕，倪灵，戴红卫,万朝霞，钟晓波 （重庆医科大学附属口腔医院牙体牙髓科 重庆 400015）） 【摘要】目的:探讨糖尿病在大鼠实验性牙周炎发生发展过程中的作用。方法：48只雄性SD大鼠采用在右上颌第一磨牙牙周拴丝的方法构建大鼠牙周炎模型。 随机均分为二组，每组24只，糖尿病+牙周炎组（G1），牙周炎组(G2)，其中G2组左上颌第一磨牙作为空白对照组(G3)，G1组大鼠在腹腔内注射链脲佐菌素（streptozotocin,STZ）建立糖尿病模型。分别在建模后第2、3、4、5周每组（G1，G2）随机处死6只大鼠，测量血糖、探诊深度、牙槽骨丧失高度、免疫组化检测牙周组织中IL-1β，TNF-α的表达和进行牙周形态组织学的评价。结果：1、同一时间点,血糖G1G2（G3），组间比较具有统计学差异(P0.05);2.探诊深度G1G2G3，每两组间比较具有统计学意义（P0.05）;3.牙槽骨丧失高度G1组显著大于G2和G3组，具有统计学差异（P0.05）;4.G1组牙周组织IL-1β，TNF-α的表达最明显，其次是G2，再次是G3，G1G2G3,各组间有统计学差异;5.G1组牙周形态组织炎症表现最明显，表明牙周炎症进展较G2，G3组快速。结论：糖尿病可以使大鼠牙周病变进一步恶化。 关键词：糖尿病；牙周炎；牙槽骨丧失 中图分类号：R781 口腔内科学 The influence of diabetes on periodontitis model in rats Deng Jie, Ni Ling, Dai Hongwei, Wan Zhaoxia, Zhong Xiaobo （The Affiliated Hospital of Stomatology, Chongqing Medical University. Chongqing.400015.China） 【Abstract】Objective: To research the influence of diabetes on periodontitis model in rats. Methods: To establish the animal model of periodontitis, 48 male SD rats received stainless steel wire ligating the right maxillary first molar. Then they were divided into two groups randomly: periodontitis combined with diabetes（G1）, periodontitis（G2),and later groups left maxillary first molar as control groups.Diabetes were builded by intraperitoneal injection of streptozocin(STZ). 6 rats were sacrificed by [基金项目]：重庆市教委科学技术研究项目基金资助课题 （KJ090316） decapitation in each group after 2, 3, 4, 5 weeks ,blood glucose concentration,probing depth（PD）and alveolar bone loss(ABL) were measured.IL-1β、TNF-α were analysed by immunohistochemistry methods. Periodontal morphology and hyphology assessed by microscope.Results:(1)The blood glucose: G1 is higher than the G2(G3), groups was statistically significant (P0.05); (2)PD:G1 is the highest,followed by G2,then G3, comparison between groups was statistically different(P0.05);(3)ABL:first G1,second G2,last G3,there are significantly differences compared to each other (P0.05).；(4)IL-1β and TNF-