冻干保护剂影响植物乳杆菌代谢途径的研究-现代食品科技.PDF

现代食品科技 Modern Food Science and Technology 2016, Vol.32, No.8 冻干保护剂影响植物乳杆菌代谢途径的研究 赵延胜，肖香，周兴华，苏萍，董英 （江苏大学食品与生物工程学院，江苏镇江 212013） 摘要：本文利用代谢组学方法，对比研究了添加不同冻干保护剂对植物乳杆菌 dy-1 （L.P dy-1 ）冻干菌粉发酵过程中代谢途径的 影响。研究采用气质联用技术（GC-MS ）分析菌体胞内代谢物，通过偏最小二乘法（PLS ）和模式识别分析不同冻干保护剂组的差异 代谢物，并结合 KEGG 等数据库对差异变量参与的代谢途径进行解析。结果表明，分别添加 10%菊粉、22.4%菊粉、菊粉复合保护剂 和脱脂乳复合保护剂，对 L.P dy-1 冻干粉发酵过程中的能量代谢、氨基酸代谢和脂肪酸代谢的影响存在差异：菊粉的添加量由 10% 增加至 22.4% ，冻干菌粉与直接活化菌体在发酵过程中的代谢特征差异缩小；与脱脂乳复合保护剂相比，菊粉复合保护剂能够使冻干 菌体在代谢特征上更接近于直接活化菌体。贮藏温度对冻干菌粉的代谢影响显著：与4 ℃相比，-20 ℃贮藏冻干菌粉更有利于维持菌 体在发酵过程中的正常代谢。 关键词：植物乳杆菌；发酵；代谢；代谢产物；冻干保护剂；气相色谱-质谱 文章篇号：1673-9078(2016)8-103-108 DOI: 10.13982/j.mfst.1673-9078.2016.8.016 Influence of Cryoprotectants on the Metabolic Pathways of Lactobacillus plantarum ZHAO Yan-sheng, XIAO Xiang, ZHOU Xing-hua, SU Ping, DONG Ying (School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China) Abstract: The effects of different cryoprotectants on the metabolic pathways of freeze-dried Lactobacillus plantarum dy-1 (L. P dy-1) powder during fermentation were analyzed through a metabolomics study. The intracellular metabolites of L. P dy-1 were separated and analyzed by gas chromatography-mass spectrometry. The metabolites produced in the presence of the different cryoprotectantss were identified and analyzed by using pattern recognition and partial least squares methods, and were employed to reconstruct the metabolic pathways using the Kyoto Encyclopedia of Genes and Genomes database. The results indicated that 10% inulin, 22.4% inulin, inulin composite protective agent, or skim milk composite protective